0001
    1 ILLINOIS POLLUTION CONTROL BOARD
    2 IN THE MATTER OF:
    )
    WATER QUALITY STANDARDS AND
    ) R08-09
    3 EFFLUENT LIMITATIONS FOR THE ) (Rulemaking-
    CHICAGO AREA WATERWAY SYSTEM ) Water
    4 AND THE LOWER DES PLAINES
    )
    RIVER: PROPOSED AMENDMENTS
    )
    5 TO 35 Ill. Adm. Code Parts 301, )
    302, 303 and 304
    )
    6
    7
    REPORT OF THE PROCEEDINGS held in the
    8 above entitled cause before Hearing Officer Marie
    9 Tipsord, called by the Illinois Pollution Control
    10 Board, taken by Steven Brickey, CSR, for the State
    11 of Illinois, 100 West Randolph, Chicago, Illinois,
    12 on the 23rd day of September, 2008, commencing at
    13 the hour of 9:00 a.m.
    14
    15
    16
    17
    18
    19
    20
    21
    22
    23
    24
    0002
    1
    A P P E A R A N C E S
    2 MS. MARIE TIPSORD, Hearing Officer
    MS. ALISA LIU, Environmental Scientist
    3 MR. ANAND RAO, Senior Environmental Scientist
    MR. TANNER GIRARD, Acting Chairman
    4 MR. JOHNSON
    MR. NICHOLAS MELAS
    5
    ILLINOIS ENVIRONMENTAL PROTECTION AGENCY
    6 1021 North Grand Avenue East
    P.O. Box 19276
    7 Springfield, Illinois 62794-9276
    (217) 782-5544
    8 BY: MS. DEBORAH WILLIAMS
    MS. STEPHANIE DIERS
    9
    MR. ROBERT SULSKI
    MR. SCOTT TWAIT
    10
    MR. HOWARD ESSIG
    11 BARNES & THORNBURG
    BY: MR. FREDRIC P. ANDES
    12 One North Wacker Drive
    Suite 4400
    13 Chicago, Illinois 60606
    (312) 357-1313
    14
    Appearing on behalf of the Metropolitan
    Water Reclamation District
    15

    DR. ERNEST BLATCHLEY III
    16
    NATIONAL RESOURCES DEFENSE COUNCIL
    17 MS. ANN ALEXANDER
    18 THE CHICAGO LEGAL CLINIC
    BY: MR. KEITH HARLEY
    19 2938 East 91st Street
    Chicago, Illinois 606017
    20 (773) 731-1762
    21 ENVIRONMENTAL LAW AND POLICY CENTER
    33 East Wacker Drive
    22 Suite 1300
    Chicago, Illinois 60601
    23 (312) 795-3707
    BY: MR. ALBERT ETTINGER
    24
    MS. JESSICA DEXTER
    0003
    1 OPENLANDS
    BY: MS. STACY MEYERS-GLEN
    2 24 East Washington Street
    Suite 1650
    3 Chicago, Illinois 60602
    (312) 863-6265
    4
    FRIENDS OF THE CHICAGO RIVER
    5 BY: MS. MARGARET FRISBIE
    28 East Jackson Boulevard
    6 Suite 1800
    Chicago, Illinois 60604
    7 (312) 939-0490
    8 OFFICE OF THE ATTORNEY GENERAL - STATE OF ILLINOIS
    BY: MS. SUSAN HEDMAN
    9 69 West Washington Street
    Suite 1800
    10 Chicago, Illinois 60602
    (312) 814-4947
    11
    12
    13 REPORTED BY:
    14
    Steven J. Brickey, CSR
    CSR License No. 084-004675
    15
    16
    17
    18
    19
    20
    21
    22
    23
    24
    0004
    1
    MS. TIPSORD: Good morning. My name
    2 is Marie Tipsord and I've been appointed by this
    3 board to serve as hearing officer in this
    4 proceeding entitled Water Quality Standards and
    5 Effluent Limitations for the Chicago Area Waterway

    6 System and Lower Des Plaines River proposed
    7 amendment 35 IL Adm. Code 301, 302, 303 and 304.
    8 The docket number is R08-9. To my immediate right
    9 is Dr. Tanner Girard, the lead board member
    10 assigned to this matter. Also present, to my far
    11 left is board member Thomas Johnson. To my
    12 immediate left Anand Rao and to his left Alisa Liu
    13 from our technical staff.
    14
    This is fifth set of hearings to
    15 be held in this proceeding and the purpose of
    16 today's hearing is to continue hearing testaments
    17 from the participants, other than the proponent,
    18 the IEPA. At the close of the hearing on
    19 September 10th, 2008, we had finished with six
    20 witnesses from the Metropolitan Water Reclamation
    21 District of Greater Chicago, the District.
    22
    We will continue with the
    23 District starting with Earnest Blatchley. Am I
    24 pronouncing that correctly, Mr. Blatchley?
    0005
    1
    MR. BLATCHLEY: Yes.
    2
    MS. TIPSORD: And then we'll go to
    3 Samuel Dorevitch, is that correct?
    4
    MR. ANDES: Yes.
    5
    MS. TIPSORD: And so on from there
    6 according to the list, the amended list filed on
    7 last Thursday, which whatever date that was. I'm
    8 drawing a blank. Sorry. The testimony will be
    9 marked as an exhibit and entered as if read. We
    10 will then immediately proceed to questions for the
    11 testifiers beginning with the Natural Resource
    12 Defense Counsel, then the IEPA, then the people,
    13 Openlands, and finally the Environmental Law and
    14 Policy Center.
    15
    Anyone may ask a follow-up
    16 question. You need not wait until your turn to
    17 ask questions. I do ask that you raise your hand,
    18 wait for me to knowledge you. After I have
    19 acknowledged you, please state your name and whom
    20 you represent before you begin your questioning.
    21 Please speak one at a time. If you're speaking
    22 over one another, the court reporter will not to
    23 able to get your questions on the record. Also
    24 note that any questions asked by a board member or
    0006
    1 staff are intended to build a complete record for
    2 the boards' decision and not to address any
    3 preconceived notion or bias. Same as last time.
    4 We're going to go until about 5:00 today. We'll
    5 take a lunch break, along with breaks throughout
    6 the day. A reminder, tomorrow, we are in 2025.
    7 That's good news and bad news. The good news is
    8 you don't have to go through security. The bad
    9 news is the rooms acoustics are even worse than
    10 this room. And with that, Dr. Girard.
    11
    MR. GIRARD: Good morning. On
    12 behalf of the board, I welcome everyone to hearing

    13 day number 15 in this water rulemaking. We are
    14 grateful for your time and contribution to this
    15 activity. We look forward to the testimony and
    16 questions today. Thank you.
    17
    MS. TIPSORD: And with that, we'll
    18 go to Mr. Andes for the District.
    19
    MR. ANDES: Yes. Thank you. Before
    20 we get into testimony, we do have some documents
    21 to provide for the record responsive to the
    22 requests that were made in the last round of
    23 hearings. And I'll walk through each of them and
    24 then I can provide copies.
    0007
    1
    The first and I think this was
    2 Environmental Law and Policy Centers request for
    3 lease documents. We provided documents with
    4 regard to one property that the District leases
    5 where there are recreational uses. It's actually
    6 a series of documents, an initial lease agreement
    7 and subsequent amendments so we have that.
    8
    MR. ETTINGER: It was Openlands that
    9 requested that. I hate reading contracts. That's
    10 why I went into litigation.
    11
    MR. ANDES: Point taken. The second
    12 document we have been asked for is information
    13 about effluent levels at Hanover Park, Egan and
    14 Kirie Treatment Plants and we've provided a table
    15 summarizing effluent data during the recreational
    16 season. Third, we were asked for copies of the
    17 raw data sheets from Geosyntec from the risk
    18 assessment and that is voluminous. We have
    19 provide that on a disc.
    20
    Next, is we were asked for any
    21 relevant citations in terms of the EPA's reliance
    22 on studies in developing water quality criteria
    23 for bacteria and for that we have a copy of the
    24 EPA's ambient water quality criteria for bacteria,
    0008
    1 a 1986 document.
    2
    And then, finally, during
    3 Dr. Tolson's (phonetic) testimony, he had
    4 described particularly two person jet skis and I
    5 was searching for the photos at the time that we
    6 were referring to. I have located those photos
    7 and we have copies for the record of the two
    8 person jet ski that he was speaking of. So those
    9 are the documents and we have multiple copies
    10 here. I'll be glad to -- I can take one copy out
    11 for the record.
    12
    MS. TIPSORD: Actually, if I could
    13 get at least two.
    14
    MR. ANDES: Sure.
    15
    MS. TIPSORD: Three if you have
    16 them. That would be great.
    17
    MR. ANDES: That's one, two, three.
    18
    MS. TIPSORD: Thank you.
    19
    MR. ANDES: This is a disc. One,

    20 two, three. Three of the lease agreements.
    21
    MS. TIPSORD: Thanks.
    22
    MR. ANDES: And I'll provide those
    23 to each and everybody that want copies of those.
    24
    MS. TIPSORD: Okay. With that, we
    0009
    1 will start with the lease agreement. It's
    2 entitled Lease Amendment Agreement Ronan Park
    3 Expansion. I will mark that as Exhibit 83 if
    4 there's no objection, seeing none, it's Exhibit
    5 83. Next, is a summary of the recreational season
    6 chlorinated/dechlorinated effluent chloroform May
    7 1 through October 21st. If there's no objection,
    8 I'll mark that as Exhibit 84. Seeing none, that's
    9 Exhibit 84.
    10
    Next, is the CD ROM raw data.
    11 I'll mark that as Exhibit Number 85, if there's no
    12 objection. Seeing none, it's Exhibit 85. And
    13 then an USEPA document Ambient Water Quality
    14 Criteria for Bacteria, 1986. I'll mark that as
    15 Exhibit 86, if there's no objection.
    16
    MS. WILLIAMS: I would just like to
    17 point out for the record it's already Attachment Q
    18 to the statements of reasons. I mean it hasn't
    19 been entered as an exhibit so I don't have an
    20 objection as to making it an exhibit, but it is
    21 already part of the record.
    22
    MS. TIPSORD: Okay. Thank you.
    23 We'll mark this as Exhibit 86. And, finally, the
    24 picture of the two person jet ski we'll mark as
    0010
    1 Exhibit 87, if there's no objection. Seeing none,
    2 it's Exhibit 87.
    3
    MR. ANDES: If I could add just to
    4 complete the picture, a couple more things.
    5
    MS. TIPSORD: Go ahead.
    6
    MR. ANDES: A couple of issues arose
    7 in terms of questions on the risk assessment.
    8 First, the distance between various pumping
    9 stations and sampling locations and we have a
    10 letter from Geosyntec to the District clarifying
    11 those locations -- those distances. And then
    12 there were also some corrections that needed to be
    13 made in terms of particular distances in the
    14 report that were inconsistent between two pages
    15 and those corrections have been sent to the
    16 District and I have both a letter from Geosyntec
    17 to the District with those corrections on page 13
    18 of the risk assessment report and a cover letter
    19 from the District to Illinois EPA enclosing those
    20 corrections.
    21
    MS. TIPSORD: Okay.
    22
    MR. ANDES: There are three copies
    23 of each.
    24
    MS. TIPSORD: We'll mark the
    0011
    1 Geosyntec consultants letter dated September 12th,

    2 2008, corrected page 13 is the subject, as Exhibit
    3 88, if there's no objection. Seeing none, it's
    4 Exhibit 88.
    5
    MS. WILLIAMS: Can I just, again,
    6 say for the record, Marie, this letter was dated
    7 yesterday. So obviously it hasn't actually been
    8 received.
    9
    MS. TIPSORD: You're speaking of the
    10 next couple of letters, not the letter I'm marking
    11 right now.
    12
    MS. WILLIAMS: Which letter did you
    13 mark?
    14
    MS. TIPSORD: The September 12th
    15 letter.
    16
    MS. WILLIAMS: Sorry.
    17
    MR. TIPSORD: That's okay. And it's
    18 noted for the record on the next one, which is
    19 September 22nd, but we'll do the Geosyntec first
    20 marked September 22nd and we'll mark that as
    21 Exhibit 89. If there's no objection, that's
    22 Exhibit 89. And then, finally, the letter to
    23 Marshal Wilhite from the District dated September
    24 22nd, which the agency has obviously not yet seen,
    0012
    1 we'll mark as Exhibit 90, if there's no objection.
    2 Seeing none, it's Exhibit 90. Speeding towards
    3 100 exhibits. Okay. Mr. Andes, anything else?
    4
    MR. ANDES: One more. Rain gauge
    5 data was requested for 2005 and 2006. I have that
    6 here. I have two copies --
    7
    MS. TIPSORD: Okay.
    8
    MR. ANDES: -- of this assemblage.
    9 And I don't remember who asked for this. It might
    10 have been the state.
    11
    MS. TIPSORD: Then we'll mark this
    12 whole group of rain gauge data as one exhibit and
    13 that will be Exhibit 91. And I have one, two,
    14 three, four, five, six paperclipped and then one
    15 big clipped grouping here. If there's no
    16 objection, we'll mark this as Exhibit 91. Seeing
    17 none, it's marked as Exhibit 91.
    18
    MR. ANDES: Let me clarify. Does
    19 the state possibly have the 2006 data only or
    20 2005? I may have --
    21
    MS. TIPSORD: I have 2005 data here.
    22
    MR. ANDES: So you have six copies?
    23
    MS. WILLIAMS: We only have 2006
    24 here.
    0013
    1
    MR. ANDES: She has six copies of
    2 2006.
    3
    MS. TIPSORD: Okay. So I only needs
    4 one of these.
    5
    MR. ANDES: Right. And then one of
    6 these.
    7
    MS. TIPSORD: Then let's clarify.
    8 Exhibit 91 is rain gauge data from 2005, the

    9 entire year. So there are 12 pages here and
    10 that's Exhibit 91. Exhibit 92 will be rain gauge
    11 data from 2006, also, for the entire year so it's
    12 12 pages, approximately. And those are both
    13 marked. And anything else, Mr. Andes?
    14
    MR. ANDES: I think that's it.
    15
    MS. TIPSORD: Okay. That would be
    16 wonderful. In that case, would you like to
    17 introduce your witness and we'll have him sworn
    18 in.
    19
    MR. ANDES: Surely. I have a copy.
    20
    MS. TIPSORD: Yes. If I could have
    21 a clean copy of his document.
    22
    MR. ANDES: This is voluminous so we
    23 put it on a disk. We have testimony, an initial
    24 copy of the testimony and then the rest is all on
    0014
    1 a disk.
    2
    MS. TIPSORD: Okay. In that case,
    3 I'm trying to think. What I'm going to do is mark
    4 both the disc and the testimony as one exhibit for
    5 purposes of citation later in the record. It
    6 could get quite difficult if we use two different
    7 exhibit numbers. So the pre-file testimony --
    8 Well, let's swear him in first.
    9 WHEREUPON:
    10
    DR. ERNEST BLATCHLEY III
    11 called as a witness herein, having been first duly
    12 sworn, deposeth and saith as follows:
    13
    E X A M I N A T I O N
    14
    MS. TIPSORD: We will mark
    15 Mr. Blatchley's pre-file testimony and attachment
    16 on a CD ROM as Exhibit 93, if there's no
    17 objection.
    18
    MS. WILLIAMS: Can I just ask a
    19 question? I think we have everything. You said
    20 it's voluminous, but this is all I have. Does
    21 that seem right to you? When we're talking about
    22 his testimony, there's his testimony, there's an
    23 expanded testimony, there's an article. I just
    24 want to make sure that I've got everything.
    0015
    1
    MS. TIPSORD: I also have very --
    2 this is it.
    3
    MS. WILLIAMS: Okay. But that's all
    4 that's on that CD. Okay.
    5
    MR. ANDES: Yes. I just thought it
    6 was easier that way.
    7
    MS. WILLIAMS: I don't think so, but
    8 I understand.
    9
    MS. TIPSORD: Okay. We'll mark that
    10 as Exhibit 93. Okay. And with that, I believe
    11 the first questions then go to the Natural
    12 Resource Defense Counsel. Ms. Alexander.
    13
    MS. ALEXANDER: Good morning,
    14 Dr. Blatchley. My name Ann Alexander. I'm from
    15 the Natural Resource Defense Counsel and I'll be

    16 asking you questions this morning --
    17
    MR. BLATCHLEY: Good morning.
    18
    MS. ALEXANDER: -- based on the
    19 pre-filed questions, which I think you have.
    20 Let's turn to the first question that I have for
    21 you, which is, do you have any formal training in
    22 the field of microbiology?
    23
    MR. BLATCHLEY: As a student, both
    24 undergraduate and graduate, I took a few classes
    0016
    1 that relate to microbiology, but I am not a
    2 microbiologist.
    3
    MS. TIPSORD: Mr. Blatchley, you're
    4 going to have to speak up.
    5
    MS. ALEXANDER: Would you say that
    6 you worked with microbiological data fairly
    7 frequently in the context of your research
    8 concerning disinfection engineering?
    9
    MR. BLATCHLEY: Yes.
    10
    MS. ALEXANDER: Okay. So would it
    11 be fair to say that you have a working knowledge
    12 of microbiology, but you're not a specialist in
    13 it?
    14
    MR. BLATCHLEY: Yes.
    15
    MS. ALEXANDER: Did you participate
    16 in any manner in the microbial risk assessment
    17 that was conducted by Geosyntec for the Water
    18 Reclamation District?
    19
    MR. BLATCHLEY: No.
    20
    MS. ALEXANDER: Have you reviewed
    21 that?
    22
    MR. BLATCHLEY: Yes.
    23
    MS. ALEXANDER: Did you provide any
    24 comments on it of any kind?
    0017
    1
    MR. BLATCHLEY: Do you mean to
    2 Geosyntec in their preoperation of the report?
    3
    MS. ALEXANDER: Geosyntec or the
    4 District.
    5
    MR. BLATCHLEY: With respect to
    6 their preparation of the report or just comments
    7 after I read it?
    8
    MS. ALEXANDER: Either one.
    9
    MR. BLATCHLEY: I think we may have
    10 had some discussion afterwards, but, honestly, I
    11 don't recall.
    12
    MS. ALEXANDER: Okay. Do you recall
    13 at all the nature of the discussions that you had?
    14
    MR. BLATCHLEY: No. I'm sorry. I
    15 don't.
    16
    MS. ALEXANDER: Have you performed
    17 any research yourself specifically for the
    18 District? I'm not referring to your testimony,
    19 but research for the District.
    20
    MR. BLATCHLEY: When you say for the
    21 District, what do you mean?
    22
    MS. ALEXANDER: Have you been

    23 retained by the District to perform any research?
    24
    MR. BLATCHLEY: No.
    0018
    1
    MS. ALEXANDER: All right. I would
    2 like to turn to your pre-filed testimony, which --
    3 I'm sorry -- was Exhibit --
    4
    MR. TIPSORD: 93.
    5
    MS. ALEXANDER: -- 93 and I'd like
    6 to turn to page three, please, under the large
    7 heading Problems with Proposed Effluent Bacteria
    8 Limit and then under the subheading, coliform
    9 bacteria are poor indicators of disinfection
    10 ethiticity. I just want to read a little language
    11 into the record, but I would like to ask you some
    12 questions about it.
    13
    MR. ANDES: I'm sorry. What page
    14 are we on?
    15
    MS. ALEXANDER: We're on page three
    16 under the subheading regarding coliform bacteria.
    17
    MS. TIPSORD: Ms. Alexander, are you
    18 asking question number two?
    19
    MS. ALEXANDER: Yes. I'm sorry.
    20 This is question number two.
    21
    MS. TIPSORD: It might help if you
    22 identify the question.
    23
    MS. ALEXANDER: I'm sorry. Yes.
    24 The language in your testimony is, for some common
    0019
    1 pathogens, analytical methods for measurement of
    2 their concentration do not exist or are difficult
    3 to perform. The large number of microbial species
    4 that can be found in municipal waste water also
    5 complicate quantification of potential microbial
    6 pathogens. From a practical perspective, it is
    7 impossible to measure the concentrations of all
    8 pathogens in waste water.
    9
    As an alternative, it is common
    10 to measure the concentration of available and/or
    11 infected indicators organisms in water. So my
    12 first question would be, does this basically
    13 define the reason in your view that indicator
    14 bacteria are commonly used to estimate or to
    15 estimate the presence of pathogens? Pathogens
    16 levels, I should say.
    17
    MR. BLATCHLEY: My view is that
    18 indicator organisms are just that, an indicator of
    19 the presence of pathogens. Coliform bacteria, are
    20 you asking specifically about them?
    21
    MS. ALEXANDER: I'm asking, first,
    22 more broadly about indicator organisms. I mean I
    23 should ask the foundational question. What do you
    24 consider to be in the category of indicator
    0020
    1 organisms?
    2
    MR. BLATCHLEY: Coliform bacteria,
    3 and/or cocci. There have been people who
    4 suggested the use of a total bacterial count.

    5 Some people have suggested the use coliphage.
    6
    MS. ALEXANDER: Are either total
    7 bacteria count or coliphage in use as in any
    8 context that you're aware of?
    9
    MR. BLATCHLEY: No. Not that I'm
    10 aware of.
    11
    MS. ALEXANDER: Okay. So the ones
    12 that are in use would be the coliform and the
    13 enterococcus?
    14
    MR. BLATCHLEY: I believe so, yes.
    15
    MS. ALEXANDER: So when you referred
    16 to indicator bacteria in your testimony, are you
    17 basically referring to coliform enterococci?
    18
    MR. BLATCHLEY: Coliforms.
    19
    MS. ALEXANDER: Coliforms. Yes.
    20 Okay. So my question, my initial question simply
    21 is, would you consider the statement that I just
    22 read into the record to essentially explain the
    23 reason why indicator bacteria are commonly used to
    24 estimate pathogen concentrations?
    0021
    1
    MR. BLATCHLEY: Yes, I think that's
    2 the idea.
    3
    MS. ALEXANDER: Okay. Would you
    4 agree then that indicator bacteria can be a good
    5 indicator of the presence of at least some types
    6 of pathogens?
    7
    MR. BLATCHLEY: Yes.
    8
    MS. ALEXANDER: Okay. I would like
    9 to turn to the third page of your extended
    10 testimony, which unfortunately is unnumbered, but
    11 the third page of it starts with the words "the
    12 concept of an indicator organism," and then
    13 there's some bullet points.
    14
    Going to the paragraph below
    15 that, which begins although and I'll just read
    16 that language into the record. Although, no
    17 organism has been identified, but ideally or
    18 completely satisfies these criteria, as referring
    19 to the criteria listed for a good indicator
    20 organism, a number of bacterial species have been
    21 proposed to satisfy this function. Commonly used
    22 indicators include coliform bacteria, e-coli and
    23 enterococci. My question there is, would you say
    24 that coliform and enterococci are essentially the
    0022
    1 best indicators available in use now?
    2
    MR. BLATCHLEY: Those are two
    3 questions.
    4
    MS. ALEXANDER: You're right.
    5 That's two separate questions. Let me ask the one
    6 about in use. Are they the best in use now?
    7
    MR. BLATCHLEY: By default, they're
    8 basically the only ones in use.
    9
    MS. ALEXANDER: Okay. And would you
    10 say that they're wildly used now?
    11
    MR. BLATCHLEY: Yes.

    12
    MS. ALEXANDER: For what sorts of
    13 purposes?
    14
    MR. BLATCHLEY: Monitoring of waste
    15 water effluent microbial quality.
    16
    MS. ALEXANDER: And are they also
    17 used to make other types of determinations such as
    18 closure of beaches?
    19
    MR. BLATCHLEY: I believe so, yes.
    20
    MS. ALEXANDER: Okay.
    21
    MR. ANDES: I'd like to follow up on
    22 that. Dr. Blatchley, can you explain a little bit
    23 more? Are we talking about indicators being an
    24 indicator of presence or the levels of pathogens?
    0023
    1
    MR. BLATCHLEY: The presence of
    2 pathogens is what is indicated by indicator
    3 bacteria or indicator organisms, more generally.
    4
    MS. ALEXANDER: Okay. Let me follow
    5 up on that. Is it your understanding that
    6 indicator bacteria are usually used to signal in
    7 some manner a threshold level above which some
    8 action is required either closing a beach or
    9 disinfection?
    10
    MR. BLATCHLEY: I believe that is
    11 the approach that is used for purposes of defining
    12 beach closures, yes.
    13
    MS. ALEXANDER: So in other words,
    14 would it be fair to say that in that regard
    15 indicator bacteria are used to signify a level in
    16 the sense that they set that threshold?
    17
    MR. BLATCHLEY: Well, I'm not
    18 involved in those decisions myself so I have to
    19 plead ignorance.
    20
    MS. ALEXANDER: I understand.
    21
    MR. BLATCHLEY: I assume that is the
    22 basis on which they are proceeding.
    23
    MS. ALEXANDER: Okay.
    24
    MR. ANDES: I'd like to follow up on
    0024
    1 that. From a scientific perspective, can you
    2 explain what you think those indicators tell you?
    3
    MR. BLATCHLEY: Again, the
    4 indicators indicate the presence or the possible
    5 presence of microbial pathogens. They don't
    6 necessarily indicate the absence of microbial
    7 pathogens for reasons that I'm sure we'll get
    8 into.
    9
    MS. ALEXANDER: Yes. And let me
    10 just follow up to clarify that. Am I correct in
    11 understanding that your fundamental concern as
    12 expressed in the testimony with indicator bacteria
    13 is that they are poor indicators in your view of
    14 the effectiveness of the disinfection process
    15 because they are more easily killed by
    16 disinfection than certain types of pathogens, is
    17 that correct?
    18
    MR. BLATCHLEY: Yes, that is a

    19 concern of mine.
    20
    MS. ALEXANDER: Okay. Moving to
    21 pre-file question three, is it possible to apply
    22 levels of disinfection that kill both the
    23 indicators and some or most of the microbial
    24 pathogens?
    0025
    1
    MR. BLATCHLEY: I'm glad you added
    2 that last phrase because, yes, it is possible to
    3 apply disinfection to be effective against most
    4 microorganisms, but disinfection is not the same
    5 thing as sterilization. Sterilization is
    6 effectively impractical to accomplish.
    7
    MS. ALEXANDER: Okay. Looking at --
    8 I'd like to turn to table two of your extended
    9 testimony which is headed UV Doses Required For 99
    10 Percent Inactivation.
    11
    MS. TIPSORD: Excuse me,
    12 Ms. Alexander. And for the record, his extended
    13 testimony is Attachment two to the pre-file
    14 testimony.
    15
    MS. ALEXANDER: Okay. So Attachment
    16 Two to Exhibit 93. And --
    17
    MR. ANDES: I'm sorry. Where were
    18 we in that?
    19
    MS. ALEXANDER: Table two, which
    20 should be on the sixth page of it. Am I correct
    21 in understanding that this table lists doses of UV
    22 radiation that can be applied to achieve 99
    23 percent inactivation of water bourne
    24 microorganisms?
    0026
    1
    MR. BLATCHLEY: Let me just clarify.
    2 These values -- the general answer to your
    3 question is yes. These values came from a
    4 tabulation that was assembled basically for people
    5 who are interested in UV disinfection and the
    6 values that I pulled off of here for many
    7 experiments that were conducted on -- Well, for
    8 example, with e-coli, there were many experiments
    9 that were conducted where values were reported.
    10 So the values that I'm listing here are values
    11 that were reported independently by many
    12 investigators. Is that clear?
    13
    MS. ALEXANDER: I think so. So are
    14 you saying that these are essentially the most
    15 accurate numbers that you could come up with based
    16 on the research for purposes of your extended
    17 testimony?
    18
    MR. BLATCHLEY: No. I would say
    19 these are the available numbers that I came up
    20 with. There was no attempt on my part to identify
    21 the quality of the numbers associated. They were
    22 just simply recording of values that they,
    23 themselves, had previously been recorded.
    24
    MS. ALEXANDER: So are you saying
    0027

    1 then that you didn't review all of the underlying
    2 research that resulted in the data that's
    3 presented in table two?
    4
    MR. BLATCHLEY: That's correct.
    5
    MS. ALEXANDER: Okay. Do you have
    6 any reason to believe that the UV doses that are
    7 identified here as necessary to achieve 99 percent
    8 inactivation of water bourne pathogens are in any
    9 way not technology feasible as a general matter?
    10
    MR. BLATCHLEY: Just to clarify, are
    11 you asking is it possible to develop UV systems
    12 that will deliver this amount of radiation?
    13
    MS. ALEXANDER: That's correct.
    14
    MR. BLATCHLEY: Sure.
    15
    MS. ALEXANDER: Okay. Are any such
    16 UV systems in use that you're aware of?
    17
    MR. BLATCHLEY: Yes, many.
    18
    MS. ALEXANDER: Do you have any
    19 reason to believe one way or the other that it
    20 would not also be possible to use such a system at
    21 the District, at the District's water treatment
    22 plant?
    23
    MR. BLATCHLEY: I believe it would
    24 possible, yes.
    0028
    1
    MS. ALEXANDER: Okay.
    2
    MR. ANDES: I would like to follow
    3 up on that. Can you compare the kinds of systems
    4 that would be required to meet the proposed
    5 standards?
    6
    MR. BLATCHLEY: What do you mean?
    7
    MR. ANDES: If we're talking about
    8 UV doses required to meet these kind of numbers,
    9 is that --
    10
    MR. BLATCHLEY: Where would we be
    11 within this range, is that what you're talking
    12 about?
    13
    MR. ANDES: Well, are we talking
    14 about systems that are more expensive than what
    15 would be required under this proposal?
    16
    MR. BLATCHLEY: I'm still confused
    17 by your question. I'm sorry.
    18
    MR. ANDES: Let's keep going.
    19
    MS. ALEXANDER: Pre-filed question
    20 number four, what is the alternative to the use of
    21 coliform bacteria and enterococci as an indicator
    22 of disinfection effectiveness? I believe that's
    23 partially been asked and answered, but I'll put it
    24 out anyway because I'm not entirely sure.
    0029
    1
    MR. BLATCHLEY: Okay. You could use
    2 other organisms and I've identified a few total
    3 bacterial counts or coliphage as an example. You
    4 could also accompany those requirements with
    5 requirements on the characteristics of the
    6 disinfection system, meaning if -- For example, UV
    7 is used, how much UV is applied, what the

    8 characteristics of the water are that come into
    9 the UV system. All of those could be
    10 incorporated.
    11
    MS. WILLIAMS: Dr. Blatchley, can
    12 you just explain to me quickly when we're talking
    13 about coliform bacteria here, are you talking
    14 about total when you're using that term, total
    15 coliform?
    16
    MR. BLATCHLEY: I didn't get that.
    17
    MS. TIPSORD: Ms. Williams, you're
    18 going to have to speak up.
    19
    MS. WILLIAMS: We've been using the
    20 word coliform in Dr. Blatchley's testimony quite a
    21 bit and I think I want to just understand whether
    22 we're talking about coliform or fecal coliform.
    23
    MR. BLATCHLEY: The data, for
    24 example, in table two that we just talked about,
    0030
    1 refers specifically to e-coli, which is a species
    2 of coliform bacteria. The majority of the data in
    3 the reports that I referred to refer to fecal
    4 coliform bacteria, which is related, but not
    5 identical. Does that answer your question?
    6
    MS. WILLIAMS: I think so.
    7
    MR. BLATCHLEY: Okay.
    8
    MS. ALEXANDER: Getting back to your
    9 testimony just now regarding the possibility of
    10 using the UV level essentially as an indicator of
    11 microbial destruction, is that method in use in
    12 any municipal waste water treatment system in the
    13 country that you're aware of? And I mean that --
    14 I should clarify the question. I mean without
    15 also use of indicator bacteria so solely using the
    16 UV level.
    17
    MR. BLATCHLEY: Solely using that
    18 level?
    19
    MS. ALEXANDER: Yes.
    20
    MR. BLATCHLEY: I'm not aware that
    21 it is, no.
    22
    MS. ALEXANDER: Okay.
    23
    MR. ETTINGER: I'm sorry. I'm not
    24 sure I understood Ms. Alexander's question. Can I
    0031
    1 just follow up slightly? As I understand what
    2 your suggestion was is that the standard would be
    3 written in with the technology level rather than a
    4 fecal coliform level. Am I wrong?
    5
    MR. BLATCHLEY: No, my suggestion
    6 was both.
    7
    MR. ETTINGER: Was both?
    8
    MR. BLATCHLEY: Yes.
    9
    MR. ETTINGER: So you would be more
    10 comfortable if you were trying to design a permit
    11 if it would have both a technology requirement and
    12 an, indicator requirement?
    13
    MR. BLATCHLEY: Yes.
    14
    MR. ETTINGER: Thank you.

    15
    MS. ALEXANDER: All right. Moving
    16 to question five, again, I think that's been
    17 partially answered, but perhaps not completely so
    18 let's go there. Regarding the statement in your
    19 testimony at three, that -- and I'll quote "use of
    20 coliform as an indicator organism provides
    21 potentially misleading information regarding the
    22 performance of disinfection systems." Is what you
    23 essentially mean by that that these indicators can
    24 provide, as it were, a false reassurance of
    0032
    1 safety?
    2
    MR. BLATCHLEY: Yes.
    3
    MS. ALEXANDER: Okay.
    4
    MR. ANDES: Can you explain that
    5 more fully?
    6
    MS. ALEXANDER: Okay. The concern
    7 is that coliform indicator bacteria are
    8 insufficiently protective as a measure of the
    9 presence of pathogens, is that correct, in
    10 identifying your concerns?
    11
    MR. BLATCHLEY: Yes.
    12
    MR. ANDES: Please --
    13
    MR. BLATCHLEY: Do you want me to
    14 expand?
    15
    MR. ANDES: Yes.
    16
    MR. BLATCHLEY: As we examined
    17 before, coliform bacteria are very sensitive to
    18 disinfect and exposure. So the conditions of
    19 disinfect and exposure that are required to
    20 accomplish irregulatory limits like 400 CFU's per
    21 100 ML are really fairly mild and just because you
    22 satisfy that constraint does not necessarily mean
    23 that you've inactivated the microbial pathogens
    24 that exist in the water.
    0033
    1
    MR. ANDES: And what would be
    2 required to actually inactivate those pathogens?
    3
    MR. BLATCHLEY: Well, as an example,
    4 in water reuse applications where direct human
    5 contact is likely to take place because the water
    6 is going to be used for irrigation or whatever,
    7 under those circumstances the extent of disinfect
    8 and exposure is anywhere from five to ten times
    9 greater than what would be required to meet these
    10 regulations. So, I mean depending upon the
    11 disinfectant I suppose, would be the --
    12
    MR. ANDES: And then the cost in
    13 treating would be five to ten times higher, is
    14 that correct?
    15
    MR. BLATCHLEY: As a ball park
    16 number, yes, it would be roughly five to ten times
    17 higher.
    18
    MR. ETTINGER: If I can just ask
    19 about the indicator again. Is your problem with
    20 the 400 or the fecal? I mean if you made the
    21 number 20 as opposed to 400 would that satisfy

    22 your objection or would it not have any effect?
    23
    MR. BLATCHLEY: There's several
    24 issues. One problem is the 400 because it
    0034
    1 really -- that's not really very difficult to
    2 accomplish and the conditions that are required to
    3 accomplish that are really pretty mild in terms of
    4 disinfection. So the number frankly to me seems
    5 not very effective in terms of controlling
    6 microbial pathogens. Another issue is that the
    7 waste water effluents are not the only source of
    8 pathogens to the waterways and no matter what you
    9 do to the waste water effluents, if it were
    10 theoretically possible to sterilize, that still
    11 wouldn't solve the problem.
    12
    MR. ETTINGER: Leaving aside that
    13 second problem, and we understand that that's
    14 another issue here, let's assume we had a
    15 situation here where the only source of pathogens
    16 was the waste water. Is there a number less than
    17 400 in which you would be comfortable that we did
    18 have an adequate indicator of whether or not there
    19 were pathogens in the water?
    20
    MR. BLATCHLEY: It would depend on
    21 what the intended use of the water is, but if the
    22 intended use of the water is going to be something
    23 like, you know, irrigation as is done in southern
    24 California, the limits that are applied there are
    0035
    1 basically the limits of defection for the
    2 analytical method for coliform bacteria. So it's
    3 2.2 per hundred ML base on the MPN method, which
    4 is essentially the limit of detection, but they
    5 also need to validate that they're getting four
    6 logs of inactivation of enterococcus viruses. And
    7 that's done basically by assuring that the
    8 conditions of disinfection are adequate to ensure
    9 that that's accomplished reliably.
    10
    ME. ETTINGER: That's the
    11 technology. How do you do that? Do you look at
    12 the -- do you have a technology requirement or how
    13 does that work?
    14
    MR. BLATCHLEY: Yes, I believe so.
    15 I don't think it's practical to monitor the
    16 enteric viruses. That's not going to be done. It
    17 can be done in a research setting, but to do it
    18 every day I think is just not -- I'm not aware
    19 that anybody does that, but I could be wrong.
    20
    MR. ETTINGER: So how do you monitor
    21 to make sure you're getting the enteric viruses if
    22 you're not counting the viruses themselves?
    23
    MR. BLATCHLEY: The approach that's
    24 used there is very similar to the approach that's
    0036
    1 used in drinking water where the concentration of
    2 microbial pathogens is presumably low. So, again,
    3 what you do is ensure the conditions of

    4 disinfection and the water quality approaching the
    5 disinfection are such that you would expect that
    6 an acceptable water quality would result.
    7
    MR. ETTINGER: Just so we can go and
    8 look at such a permit and see how it's done in a
    9 regulatory manner, are you familiar with any
    10 particular permit that has these sorts of
    11 conditions that you're talking about that would
    12 provide for the monitoring that you would think
    13 was adequate to protect in this irrigation
    14 situation?
    15
    MR. ANDES: We do have a copy of the
    16 compilation of the California Reuse Requirements,
    17 if that's helpful.
    18
    MR. ETTINGER: That would be
    19 something we could look at then.
    20
    MR. ANDES: Yes. I have copies.
    21
    MR. ETTINGER: Thank you.
    22
    MR. ANDES: Sure.
    23
    MS. TIPSORD: Are we going to enter
    24 that as an exhibit then?
    0037
    1
    MR. ANDES: I'm fine with that.
    2
    MR. ETTINGER: I have no objection.
    3
    MS. TIPSORD: We might get to 100
    4 today.
    5
    MR. ANDES: I think we're going to
    6 get there.
    7
    MS. TIPSORD: I will mark as Exhibit
    8 94, California Health Laws Related to Recycled
    9 Water. It's a June 2001 addition from the
    10 California -- from the purple book. If there's no
    11 objection, that's Exhibit 94. Seeing none, it's
    12 Exhibit 94.
    13
    MR. ETTINGER: Could I just follow
    14 up with one other thing? You suggested or said in
    15 your testimony that part of your looking at the
    16 level would depend on the use of the waste water
    17 and then you pointed us to the irrigation
    18 situation, are you familiar with California or
    19 what others do in the swimming water situation
    20 that you were talking about?
    21
    MR. BLATCHLEY: No. Do you mean
    22 beaches?
    23
    MR. ETTINGER: Yeah.
    24
    MR. BLATCHLEY: No, I'm not.
    0038
    1
    MR. ETTINGER: Leaving aside the
    2 irrigation situation, again, I believe you
    3 answered that you would not be comfortable using
    4 400 fecal coliform and then we would look at the
    5 use of the water and then we went to this
    6 irrigation situation, how would your answer change
    7 if we were look at swimming as opposed to
    8 irrigation?
    9
    MR. BLATCHLEY: I'm sorry. I don't
    10 know enough about what the numbers -- Presumably,

    11 the approach that would be used would be some sort
    12 of correlation between some monitoring organisms
    13 and the pathogens that you're concerned about, but
    14 I don't know the numbers that would be used under
    15 those circumstances?
    16
    MR. ETTINGER: You don't know
    17 whether you'd want to go to the detection level
    18 under those circumstances or not?
    19
    MR. BLATCHLEY: I'm sorry. I don't.
    20
    MR. ETTINGER: Thank you.
    21
    MS. TIPSORD: Mr. Harley.
    22
    MR. HARLEY: Keith Harley with the
    23 Southeast Environmental Task Force. Dr.
    24 Blatchley, you've talked about numeric limits that
    0039
    1 can appear in permits, for example, 400 coliform
    2 forming units and you've talked about approaches
    3 where you could obtain very, very low levels like
    4 2.2. With the typical application of UV systems
    5 that you've seen, what are the levels achieved in
    6 terms of the level of colony forming units in
    7 waste water?
    8
    MR. BLATCHLEY: I think they
    9 typically shoot to be reliably under the limit
    10 that is imposed. So if the limit is 400, you can
    11 expect it going to be somewhere under 400.
    12
    MR. HARLEY: Do facilities which are
    13 subject to the 400 colony forming unit numeric
    14 limit achieve better results?
    15
    MR. BLATCHLEY: Sometimes, yes.
    16
    MR. HARLEY: And what would be the
    17 best result that they would achieve using UV under
    18 typical conditions?
    19
    MR. ANDES: Can I clarify what kind
    20 of -- are you talking about conventional
    21 disinfection? He's characterized conventional
    22 disinfection versus sort of the California
    23 example. Are you talking about conventional
    24 disinfection?
    0040
    1
    MR. HARLEY: I'm talking about
    2 conventional disinfection.
    3
    MR. BLATCHLEY: I would guess there
    4 would be days where you have non-detect.
    5
    MR. ANDES: Would that be on a
    6 consistent basis?
    7
    MR. BLATCHLEY: No. There's a
    8 number of things that influence the concentration
    9 of viable coliform bacteria or any other organism
    10 that is going to leave a disinfecting system,
    11 including water quality that comes in. And that
    12 is not the same from day-to-day or even hour to
    13 hour. So it depends on, you know, when you
    14 collect your sample, what the characteristics of
    15 the treatment system upstream of disinfection were
    16 and a number of other things.
    17
    And, in fact, the analytical

    18 methods that you use to quantify micro organisms
    19 also are subject to quite a bit of error. There's
    20 a fair amount of error in those analytical methods
    21 just in the numbers that we report. So it's
    22 common to see, you know, substantial variations in
    23 those numbers. So I wouldn't be surprised to see
    24 non-detects from time to time and also things that
    0041
    1 approach or even exceed the limit from time to
    2 time in various facilities. I think that's pretty
    3 common.
    4
    MR. HARLEY: Thank you.
    5
    MS. ALEXANDER: With respect to the
    6 400 colony forming units standard that we're
    7 discussing, were that imposed in a situation such
    8 as the District, as has been proposed by IEPA,
    9 would you expect that there would be at least some
    10 reduction in the pathogen levels of the effluent?
    11
    MR. BLATCHLEY: Yes.
    12
    MS. ALEXANDER: So the question that
    13 we're addressing in your testimony is how to get a
    14 greater reduction, not whether there's going to be
    15 some reduction or no reduction, is that correct?
    16 It's level of safety?
    17
    MR. BLATCHLEY: That's one of the
    18 questions, yes.
    19
    MR. ANDES: And what are the other
    20 questions?
    21
    MR. BLATCHLEY: Again, the other
    22 concerns I have relate to what are the sources of
    23 pathogenic microorganisms that exist in the
    24 waterways. That would be the respective of what
    0042
    1 you do with the effluent that's not going to be
    2 effected by what's being proposed.
    3
    MS. ALEXANDER: Okay. We'll get to
    4 that subject a little further down. Can you
    5 explain if one had a chlorination system that was
    6 essentially designed to meet the 400 colony
    7 forming unit limit, what would have to be done to
    8 that system in order to meet a more stringent
    9 limit of the type that you discussed in your
    10 testimony?
    11
    MR. BLATCHLEY: Okay. The example
    12 that I gave in the testimony, I believe, referred
    13 to Title 22 systems in California. These are
    14 reuse systems where, again, the microbial
    15 constraints are less than 2.2 per hundred ML,
    16 which basically means non-detect and you need to
    17 demonstrate, let's say, four log units of enteric
    18 virus inactivation.
    19
    The conditions of chlorinations
    20 that are required to accomplish that, I believe,
    21 are on the order of four to five milligrams per
    22 liter of free chlorine and 120 minutes of contact
    23 time. So often times, we characterize that
    24 cholerine exposure as the product nominally of the

    0043
    1 concentration of the disinfectant and the exposure
    2 time or CT. So the CT value is going to be
    3 somewhere in the vicinity of 500 milligram minutes
    4 per liter.
    5
    MS. ALEXANDER: So in other words,
    6 if one has a chlorination/dechlorination system in
    7 operation and one wishes to meet a more stringent
    8 limit, it's not a question of adding a lot of new
    9 hardware, it's a question of increasing contact
    10 time and chlorine levels, am I understanding
    11 correctly?
    12
    MR. BLATCHLEY: Well, I believe that
    13 is a lot of new hardware, but, yes, you are
    14 talking about by one means or another increasing
    15 the chlorine exposure by a factor of ten roughly.
    16 So that can be done by, at least in theory, that
    17 can be done by increasing the contact time, by
    18 increasing the concentration of disinfectant that
    19 is maintained in the contact chamber or some
    20 combination of those things.
    21
    MS. ALEXANDER: And what's the new
    22 hardware that is involved in that?
    23
    MR. BLATCHLEY: A larger contact
    24 chamber. I would assume there may be new hardware
    0044
    1 associated with delivering more chlorine also.
    2
    MS. ALEXANDER: Same question with
    3 respect to ultra violet, if one had a system that
    4 was meeting 400 colony forming unit standard and
    5 one wanted to make that more -- wanted to meet a
    6 more stringent limit, what would need to be done?
    7
    MR. BLATCHLEY: You'll need to
    8 increase the size of the facility. I don't think
    9 it's quite as extreme as with chlorine. I would
    10 guess on the order of five times bigger and that
    11 basically means five times as many lamps or five
    12 times as much power that you can deliver in the
    13 form of germicidal UV radiation.
    14
    MS. ALEXANDER: Okay. If you're
    15 adding more power, is it necessary to add
    16 significant infrastructure other than that?
    17
    MR. BLATCHLEY: It's not just a
    18 question of electrical power, it's the lamp to
    19 deliver the power. So imagine in this room that
    20 you wanted to increase the visible light, the
    21 power of visible light in the room. You would do
    22 that by multiplying, let's say, by a factor of
    23 five. You would increase by a factor of five the
    24 number of lights that you had assuming that you
    0045
    1 were using the same lamp technology.
    2
    MS. ALEXANDER: So, essentially,
    3 what we're talking about to intensify the kill
    4 ratio as it were of ultra violet is a lot more
    5 light bulbs?
    6
    MR. BLATCHLEY: And related

    7 hardware, yes.
    8
    MS. ALEXANDER: Okay. Now, is it
    9 your view that there is some level of disinfection
    10 between the level of 400 colony forming units and
    11 the, essentially, reuse level in use in California
    12 that would be appropriate in a recreational
    13 waterway system such as the CAWS?
    14
    MR. BLATCHLEY: I suppose there
    15 could be one, but I'm not sure what it would be.
    16
    MS. ALEXANDER: Okay. So is it your
    17 view that this reuse level is appropriate for the
    18 CAWS?
    19
    MR. ANDES: I don't think he's
    20 opining on that issue.
    21
    MS. ALEXANDER: I'm sorry?
    22
    MR. ANDES: If you're asking from a
    23 risk assessment standpoint because that's not his
    24 area.
    0046
    1
    MS. ALEXANDER: But he has presented
    2 testimony all about why the current level is not
    3 appropriate and it ought to be made more
    4 stringent. So my question is --
    5
    MR. ANDES: I object to the
    6 characterization of his testimony. It should be
    7 made more stringent.
    8
    MS. ALEXANDER: I mean -- Hold on a
    9 second.
    10
    MR. ANDES: Pointing out that more
    11 stringent levels would be needed to kill most
    12 pathogens is a different issue than saying it
    13 should be made more stringent.
    14
    MS. ALEXANDER: I would point out
    15 that in the article that is attached to or made a
    16 part of Attachment two to Exhibit 93, highlighted
    17 in the conclusions is a statement considering --
    18 Well, I'll read the statement. "It is important
    19 to consider the second central question of this
    20 research, which is under circumstances where
    21 disinfection is necessary, how should it be
    22 accomplished," and hold on one second.
    23
    MR. ANDES: But he hasn't testified
    24 that disinfection would be necessary here. We
    0047
    1 have to characterize his reports. They are what
    2 they are.
    3
    MS. ALEXANDER: I'm lost in the
    4 language here. Just a moment.
    5
    MS. TIPSORD: Ms. Alexander, try
    6 rephrasing your question. I think we're spending
    7 a lot of time arguing a point that can be
    8 accomplished if you just rephrase your question.
    9
    MS. ALEXANDER: Do you have any
    10 basis to believe that the reuse standard in use in
    11 California is appropriate for use in a
    12 recreational water body such as the CAWS?
    13
    MR. BLATCHLEY: I don't know.

    14
    MS. ALEXANDER: You have no basis
    15 one way or the other?
    16
    MR. BLATCHLEY: No, I'm sorry. I
    17 don't.
    18
    MS. ALEXANDER: Okay.
    19
    MR. HARLEY: Before we go on --
    20
    MR. TIPSORD: Yes, Mr. Harley.
    21
    MR. HARLEY: Then why did you
    22 feature the California reuse standards so
    23 prominently in your pre-file testimony?
    24
    MR. BLATCHLEY: I wanted to
    0048
    1 illustrate that there's a range of disinfection.
    2 When people say you're going to use disinfection,
    3 what does that mean? In my mind, that means a
    4 number of things. It can range from nothing,
    5 which is applied many places, to fairly extensive
    6 disinfectant exposure which is applied, for
    7 example, in the case of reuse applications in the
    8 southwest, including California. So my point was
    9 to illustrate that disinfection is not a box that
    10 fits everyone. There is a range of these
    11 applications that exist all the way from zero to
    12 very extensive.
    13
    MS. WILLIAMS: Dr. Blatchley --
    14
    MS. TIPSORD: Ms. Williams, you need
    15 to project. They need to hear you back there too.
    16
    MS. WILLIAMS: Okay. Dr. Blatchley,
    17 if you don't have an opinion on what level of
    18 treatment would be necessary for recreational
    19 waters, why are you testifying that you think 400
    20 is not sufficiently stringent?
    21
    MR. BLATCHLEY: The research that
    22 we've done on waste water disinfection was based
    23 largely on systems that I labeled as conventional
    24 disinfection and I would include one that is
    0049
    1 designed to satisfy that constraint as a
    2 conventional disinfection system. Our
    3 observations of what happens to the microbial
    4 community as a result of that exposure and
    5 following that exposure suggests that it's really
    6 not very beneficial to do that and in some cases,
    7 it's actually detrimental in terms of microbial
    8 quality.
    9
    MS. WILLIAMS: Can you explain
    10 detrimental?
    11
    MR. ANDES: Do you want to use the
    12 charts?
    13
    MR. BLATCHLEY: Sure. This is going
    14 to take a minute to walk through.
    15
    MS. WILLIAMS: You know, there might
    16 be -- Do we want to save this? This might be
    17 going out of order to go down this path now.
    18
    MR. BLATCHLEY: Okay.
    19
    MS. ALEXANDER: I had a series of
    20 questions about this, but perhaps it will come up

    21 in the context of those questions, however, people
    22 want to do it.
    23
    MS. WILLIAMS: I asked the question,
    24 but I can withdraw it at this time.
    0050
    1
    MS. TIPSORD: Do you want to
    2 withdraw it?
    3
    MS. WILLIAMS: Yes.
    4
    MS. TIPSORD: Ms. Alexander, we're
    5 back to you.
    6
    MS. ALEXANDER: All right. It
    7 appears that pre-file question six and seven have
    8 been basically asked and answered at this point.
    9 So I am going to turn to pre-file question eight,
    10 which concludes -- involves the second portion,
    11 essentially, of conclusion number two on page nine
    12 of your pre-file testimony, which I believe also
    13 gets to the question that Ms. Williams asked and
    14 the statement that I'm referencing there is the
    15 response of the bacterial community to the
    16 post-disinfection environment will be influenced
    17 by bacterial repair, recovery and regrowth.
    18 Collectively, these processes may yield diminished
    19 water quality relative to a situation that
    20 disinfection is not practiced. Is that,
    21 essentially, the subject matter you were referring
    22 to just now when you said that the effects could
    23 be detrimental?
    24
    MR. BLATCHLEY: Yes.
    0051
    1
    MS. ALEXANDER: Okay. First off,
    2 Subquestion A, do all pathogenic bacteria exhibit
    3 the same response to chlorine disinfectants as
    4 fecal coliform?
    5
    MR. BLATCHLEY: No.
    6
    MS. ALEXANDER: Okay. So in other
    7 words, they don't all have the same capacity for
    8 repair and regrowth, is that correct?
    9
    MR. BLATCHLEY: I believe that's
    10 correct, yes, but we have not investigated it.
    11 Let me just further characterize. I'm assuming
    12 that is the case.
    13
    MS. ALEXANDER: Okay. So there's
    14 been no research one way or the other that you're
    15 aware of on that point?
    16
    MR. BLATCHLEY: No.
    17
    MS. ALEXANDER: Okay. If you used a
    18 higher level of chlorine disinfection at
    19 increasingly higher levels, I should say, would
    20 you expect that there could be a change in the
    21 ability of the microorganisms to repair and
    22 regrow?
    23
    MR. BLATCHLEY: Yes, I would expect
    24 that because generally it is assumed that the
    0052
    1 ability of an organism to repair and regrow
    2 depends on the extent to which it has been

    3 damaged.
    4
    MS. ALEXANDER: And same question
    5 for UV.
    6
    MR. BLATCHLEY: Yes. Same response.
    7
    MS. ALEXANDER: Subquestion C under
    8 question eight, do your findings regarding
    9 regrowth in your study apply to viruses and
    10 protozoa or just fecal chloroform bacteria?
    11
    MR. BLATCHLEY: In fact, they apply
    12 to fecal chloroform bacteria and the total
    13 bacterial counts within the samples.
    14
    MS. ALEXANDER: Now, I'd like to
    15 turn, please, to table three in your study that is
    16 from Water Environment Research, which is attached
    17 to Attachment two of Exhibit 93, which is the
    18 table I will represent that purports to display
    19 the numbers that reflect the regrowth of the
    20 bacteria. My first question there is under
    21 Subquestion D.
    22
    MS. TIPSORD: I'm sorry. I'm not
    23 even sure where you're at.
    24
    MS. ALEXANDER: There is a study
    0053
    1 attached to Attachment Two entitled Effective
    2 Water Bourne Disinfection on Water Bourne Bacteria
    3 and Viruses by --
    4
    MR. TIPSORD: That's actually
    5 Attachment Three.
    6
    MS. ALEXANDER: I'm sorry. You're
    7 right.
    8
    MR. TIPSORD: So Attachment Three,
    9 table three, which is page 87 of that article.
    10 Thank you. Sorry.
    11
    MS. ALEXANDER: Are we there?
    12
    MR. ANDES: Yes.
    13
    MS. TIPSORD: Go ahead.
    14
    MS. ALEXANDER: Dr. Blatchley, my
    15 first question there is -- I should clarify. T
    16 equals 144 is the end of the study period, is that
    17 correct, the point at which you measured regrowth?
    18
    MR. BLATCHLEY: No. In fact, we
    19 measured every day over a period of six days. So
    20 that would be the last day in the incubation
    21 period.
    22
    MS. ALEXANDER: So when I say T
    23 equals 144 as here in this table I'm referring to
    24 the last day of the incubation period and my
    0054
    1 question is were the levels at T equals 144, this
    2 last day of measurement, ever higher than the
    3 undisinfected levels that existed prior to T
    4 equals zero?
    5
    MR. BLATCHLEY: Repeat the question
    6 one more time because I want to make sure I
    7 understood it correctly.
    8
    MS. ALEXANDER: Okay. Looking at
    9 the table, I'm going to do this by example. Let's

    10 take Facility B, the one at the top. You have at
    11 the second column over from the right it states
    12 fecal coliform T equals zero, which is the point
    13 at which you began measurement, is that correct?
    14
    MR. BLATCHLEY: Yes. Actually, T
    15 equals zero in this experiment was post
    16 disinfection.
    17
    MS. ALEXANDER: Yes.
    18
    MR. BLATCHLEY: So that's when
    19 incubation started.
    20
    MS. ALEXANDER: Let's go down.
    21 Moving vertically, you have UV
    22 chlorination/dechlorination and then according to
    23 the table footnote, ORI width indicates the
    24 control sample with acidic substrates and without
    0055
    1 indicates without the substrates, but that was
    2 essentially without disinfection, is that correct?
    3
    MR. BLATCHLEY: Both of them were.
    4
    MS. ALEXANDER: So if we move across
    5 the table to these two numbers, ORI with and
    6 without, for Facility B you see what I would
    7 characterize as fairly high numbers. You have
    8 2.81 times 10 to the 5th and 2.16 times 10 to the
    9 5th, which is the fecal coliform levels in the
    10 undisinfected effluent, is that correct?
    11
    MR. BLATCHLEY: Yes.
    12
    MS. ALEXANDER: So am I correct in
    13 observing that regardless of any repair and
    14 regrowth, the numbers, the level of fecal coliform
    15 bacteria at the end of the study period at T
    16 equals 144 were always lower than the
    17 undisinfected levels, is that correct?
    18
    MR. BLATCHLEY: No. There's an
    19 example right here of where the opposite is true.
    20 Let me just clarify because I'm not sure that I'm
    21 understanding your question and actually let me
    22 just clarify the point of the experiment. The
    23 point of the experiment was to follow the dynamics
    24 of the microbial population post disinfection and
    0056
    1 to compare that with an undisinfected sample. So
    2 our interests were to evaluate how the microbial
    3 population responded to either the application of
    4 disinfection or the non-application of
    5 disinfection. In some cases when we evaluate the
    6 coliform concentration, for example, at the end of
    7 that experiment, the concentration of coliform
    8 bacteria in the undisinfected sample was actually
    9 higher than in the disinfected sample, meaning
    10 that after six days of incubation, the coliform
    11 concentration in the disinfected sample was
    12 actually higher than it was in the undisinfected
    13 system. Would it be clearer to look at the data
    14 just as an example?
    15
    MS. ALEXANDER: First, I'd like to
    16 clarify what is on this table because that's where

    17 I'm getting the understanding of your research
    18 results and I'm not quite seeing what you're
    19 saying here. What I do see is that T equal zero.
    20 When you apply, for instance,
    21 chlorination/dechlorination, you get a level of
    22 715 and then there was some regrowth and then you
    23 get 1133.
    24
    However, in the undisinfected
    0057
    1 effluent, you start out with a level of 2.81 or
    2 2.16 times 10 to the 5th and you end up with
    3 levels of 5825 and 7275 respectfully. So
    4 regardless of the regrowth that appears to happen
    5 between T equals zero from 715 to T equals 144,
    6 you have higher levels in the undisinfected
    7 samples after that amount of time and, of course,
    8 they're vastly higher than the undisinfected
    9 sample at T equals zero. Are those correct
    10 observations?
    11
    MR. BLATCHLEY: Yes.
    12
    MS. ALEXANDER: Okay. Moving down
    13 to the next one you've got for UV --
    14
    MR. BLATCHLEY: Are you talking
    15 about Facility D now?
    16
    MS. ALEXANDER: Yes. For Facility D
    17 for undisinfected you have a couple of numbers
    18 times 10 to the 5th and then you move across if
    19 you don't do anything to those you end with
    20 numbers of 2718 and 1262, respectfully, correct?
    21 That's at T equal 144 in the first column over to
    22 the right. That's your -- the level of
    23 undisinfected if you just leave it sitting in the
    24 petri dish or whatever you use to come up with
    0058
    1 that. Here, if you disinfect with
    2 chlorination/dechlorination, you appear to have
    3 some regrowth from 61.5 which is, of course, a lot
    4 lower than these undisinfected numbers and then it
    5 regrows to 20/40.
    6
    MR. BLATCHLEY: Which is higher than
    7 the 1282.
    8
    MS. ALEXANDER: Right. Which is
    9 marginally higher than the 1282.
    10
    MR. ANDES: I'd object to
    11 marginally.
    12
    MS. ALEXANDER: Is that the one
    13 example you were referring to?
    14
    MR. BLATCHLEY: Well, Facility A as
    15 well.
    16
    MS. ALEXANDER: Right. Okay.
    17 Right. There's two examples. There's a Facility
    18 A and a Facility D. So in other words, the
    19 differences that you're referring to are
    20 essentially of that order, correct, within the
    21 same order of magnitude, but there are some
    22 marginally higher numbers in these circumstances
    23 at the end of the study period in the

    24 undisinfected versus the disinfected, is that
    0059
    1 correct?
    2
    MR. ANDES: I'd object to the
    3 marginally. I'd let him characterize it himself,
    4 but he can also use the chart to talk about it.
    5
    MS. ALEXANDER: Okay. Of the same
    6 order of magnitude I would say.
    7
    MR. BLATCHLEY: I think that's a
    8 fair characterization, yes.
    9
    MS. ALEXANDER: Okay. And those are
    10 the only two examples, in that correct, in this
    11 table?
    12
    MR. ANDES: Two out of four.
    13
    MS. ALEXANDER: It not's two out of
    14 four because it's specific types of disinfection.
    15
    MR. ANDES: There's four of them and
    16 there's two.
    17
    MS. ALEXANDER: There's eight
    18 examples because in both you use UV and
    19 chlorination, correct, two different types of
    20 experiments?
    21
    MR. ANDES: There's more than two
    22 situations where they're low. The point he is
    23 trying to make is in some cases the levels after
    24 disinfection are higher than the undisinfected
    0060
    1 effluent and that point is made by the chart.
    2
    MS. WILLIAMS: Okay. Can we talk
    3 about the chart? Is this going to be an exhibit?
    4
    MR. ANDES: Yes.
    5
    MS. WILLIAMS: I thought it was a
    6 blow up of something in here, but it's not, is it?
    7
    MR. BLATCHLEY: No.
    8
    MR. ANDES: Right. And I know I do
    9 have copies of that for everyone if I can just
    10 locate them.
    11
    MS. ALEXANDER: All right. I think
    12 we're ready to go to the chart now.
    13
    MR. BLATCHLEY: Can you see it? Do
    14 you need me to move it?
    15
    MR. TIPSORD: You can tilt it this
    16 way. Turn it a little bit. And we'll wait until
    17 we get the paper.
    18
    MR. ANDES: I'm looking.
    19
    MR. BLATCHLEY: Can you see it now?
    20
    MS. ALEXANDER: Yes, I can see it.
    21
    MS. TIPSORD: We're going to wait
    22 until we get a hard copy so everyone can see it.
    23
    MR. ANDES: I'm sorry. I am unable
    24 to locate my copies, but I have copies made.
    0061
    1
    MS. TIPSORD: Okay. It's probably
    2 easier to turn it this way and we'll move down.
    3
    MR. BLATCHLEY: Is that correct?
    4 Whatever you want.
    5
    MS. TIPSORD: Just turn it this way.

    6
    MR. BLATCHLEY: Okay. So let me
    7 explain the experiment and the data and how it's
    8 being presented and then I'll kind of walk you
    9 through it. The experiment involved the
    10 collection of undisinfected samples from a number
    11 of different waste water treatment facilities,
    12 municipal waste water treatment facilities. We
    13 would have them shipped to our lab and then we
    14 would perform some form of treatment at the bench
    15 involving those samples. Now, the treatment that
    16 we would use in the case of UV or chlorine, these
    17 were disinfectant exposures, that other
    18 experiments that we had conducted, had suggested,
    19 would allow us to comply with the relevant
    20 discharge regulations. So usually it's going to
    21 be a coliform standard that we needed to meet,
    22 fecal coliform standard that we needed to meet.
    23
    So, again, what we wanted to do
    24 in these experiments was to mimic what would have
    0062
    1 been done at full scale, but do it in our lab
    2 under controlled conditions where we could then
    3 take those samples and then evaluate what happens
    4 to them chemically or microbiologically. In this
    5 case, what we did was we took those samples and we
    6 divided post disinfectant exposure, we incubated
    7 them for a period of six days.
    8
    And every day we would collect a
    9 sample, among the things we would do is collect a
    10 sample from that incubated sample and measure the
    11 total bacteria counts and the fecal coliform
    12 concentration, viable fecal coliform
    13 concentration. So for each one of these samples
    14 that we would collect from a waste water treatment
    15 facility, there would be a UV disinfected sample,
    16 a sample that was subjected to chlorination and
    17 dechlorination and both of those samples before we
    18 started the incubation, we add a little bit of
    19 acidic acid because we had determined that would
    20 be representative of the partially reduced
    21 substrates that these micro organisms might
    22 encounter when they were released to a receiving
    23 stream or something like that.
    24
    So we actually did two controls
    0063
    1 in these experiments. One control was the
    2 undisinfected sample to which we added that same
    3 substrates and that's labeled as original with and
    4 another was the undisinfected sample to which we
    5 added nothing. So that's original without. So
    6 for each sample we collect then there are four
    7 treatments that we evaluated, UV,
    8 chlorination/dechlorination, the control with a
    9 substrates and the control without the substrates.
    10 Does that make sense? It's a lot, I think.
    11
    And in each experiment what we
    12 would do, again, would be to follow the total

    13 bacterial numbers up here and the viable coliform
    14 concentration. Okay? So there's a couple of
    15 patterns that show up in this data set and I
    16 should say also that for each facility we
    17 collected samples on four different dates and
    18 subjected them to this essay. So these are
    19 actually the averages of these four data sets.
    20
    MS. TIPSORD: Excuse me,
    21 Dr. Blatchley. I remember you talking about the
    22 transcript. People aren't going to have that. So
    23 for the record, you're pointing to the chart
    24 that's labeled Facility D St. Petersburg, which
    0064
    1 we'll enter as Exhibit 95 when we get a copy of
    2 it. So when you talk about the things you're
    3 discussing, you're pointing to that chart and
    4 talking about the plotting on the chart.
    5
    MR. BLATCHLEY: Should I refer to it
    6 as Exhibit 95?
    7
    MS. TIPSORD: That's fine. I just
    8 wanted to be sure that we got that in there
    9 because you started to refer to this and that and
    10 I want to make sure that everyone knows that
    11 you're referring to Exhibit 95. Go ahead. Thank
    12 you.
    13
    MR. BLATCHLEY: So these data down
    14 here that are illustrated represent the coliform
    15 concentrations and I should point out the vertical
    16 axis of Exhibit 95 there is a break and I did that
    17 intentionally because there is a several orders of
    18 magnitude difference between the concentration of
    19 viable coliforms that we measure and the total
    20 bacteria counts that we get. And that's evident
    21 here roughly 10 to the 8th whereas down here we
    22 might be 10 to the 3rd or 10 to the 4th.
    23
    So if we were to follow the
    24 coliform counts, what we observe is that the
    0065
    1 samples without disinfection and actually they
    2 show up above the scale over here, they tend to
    3 show some die off following disinfection. I'm
    4 sorry. Following not disinfection. So starting
    5 at T equals zero. So it's unfortunate that the T
    6 equals zero sample didn't show up with this axis
    7 break, but I believe it's somewhere over here
    8 about 10 to the 5th and following the initiation
    9 of this incubation experiment, again, the
    10 concentration of these things just gradually dies
    11 and that's pretty commonly observed with coliform
    12 bacteria.
    13
    The contrast to that would be
    14 the UV disinfected sample, which is the blue dot
    15 or triangles and, I guess, it's the pink hexagon,
    16 which represents the sample that was subject to
    17 chlorination/dechlorination. Their behavior is
    18 somewhat erratic in the case of chlorine, but
    19 generally we see a trend of increasing

    20 concentration of those coliforms. And, actually,
    21 the general trend -- I'm not sure how you account
    22 or do this in your reporting, but the general
    23 trend is to have those two things converge.
    24
    And in this case, in the case of
    0066
    1 chlorination/dechlorination the concentration
    2 actually exceeded the controls at the end of the
    3 experiment. Okay? It's also important to point
    4 out what's happening with the total numbers up
    5 here. This set of inverted red triangles
    6 represent the response of the total bacterial
    7 community post disinfection with chlorine being
    8 the disinfectant and you see that after two days
    9 we have roughly an order of magnitude more
    10 bacteria than total bacteria than were present in
    11 any of the other samples.
    12
    So to clarify there was no
    13 effort that was made here to try to identify what
    14 comprises that population of bacteria. It was
    15 simply a body count with no species
    16 identification, but clearly the concentration here
    17 is higher than it is down here by roughly an order
    18 of magnitude. Does that define or does that
    19 clarify how we did those experiments and what they
    20 suggest?
    21
    MS. ALEXANDER: It's helpful and
    22 since this is the first time I have seen this
    23 chart I may need to review it and ask some follow
    24 ups, but I just want to be clear and I'm going to
    0067
    1 go to the chart myself looking at the fecal
    2 concentration, which is what I believe was
    3 discussed in your testimony, am I correct that
    4 this line with the pink dots represents the effect
    5 of chlorine disinfection, is that right?
    6
    MR. BLATCHLEY:
    7 Chlorination/dechlorination.
    8
    MS. ALEXANDER:
    9 Chlorination/dechlorination. And then this line
    10 here the with the gray triangles is essentially
    11 the undisinfected effluent, is that correct?
    12
    MR. BLATCHLEY: Correct.
    13
    MS. ALEXANDER: So what we have
    14 going on here you have the undisinfected effluent
    15 start off somewhere here off the chart .
    16
    MR. BLATCHLEY: It's not off the
    17 chart. It's off the lower break.
    18
    MS. ALEXANDER: Okay. Off the lower
    19 break. And then you have the disinfected effluent
    20 starting off down here and you have given this
    21 erratic pattern, they gradually converge at a
    22 point almost at the end of your study period here
    23 right before the six on the timeline and then they
    24 cross. So would it be fair to say that during all
    0068
    1 of the time frame prior to this convergence right

    2 before the six, in fact, the level in the
    3 undisinfected sample is higher than in the
    4 disinfected sample?
    5
    MR. BLATCHLEY: Yes, it is, but I
    6 would say at three days you're pretty close.
    7
    MS. ALEXANDER: You're pretty close,
    8 but then you get further apart again, right?
    9
    MR. BLATCHLEY: Yes.
    10
    MS. ALEXANDER: By pretty close the
    11 distance between these two, between the
    12 disinfected pink dots and at approximately time
    13 equals three days and the gray triangle at that
    14 same point is somewhat further than the distance
    15 at T equals 144, which is day six when they have
    16 converged and crossed in the other direction, is
    17 that correct?
    18
    MR. BLATCHLEY: Sure.
    19
    MS. ALEXANDER: And would it be fair
    20 to say -- can we summarize that for the vast
    21 amount of this time except for toward the end of
    22 day five leading to day six the undisinfected
    23 numbers are substantially higher than the
    24 disinfected numbers?
    0069
    1
    MR. BLATCHLEY: The undisinfected
    2 numbers are higher.
    3
    MS. ALEXANDER: Okay.
    4
    MR. TIPSORD: Mr. Harley.
    5
    MR. HARLEY: Do you retain the
    6 samples in containers in your lab, is that
    7 correct?
    8
    MR. BLATCHLEY: Yes, in an
    9 incubator.
    10
    MR. HARLEY: How big were those
    11 containers?
    12
    MR. BLATCHLEY: I believe they were
    13 one liter samples.
    14
    MR. HARLEY: And how did you account
    15 for differences, for example, that would occur if
    16 they had been discharged into a water which was
    17 flowing or a water where the samples were heavily
    18 diluted, did you account for those kinds of
    19 discharge conditions at all?
    20
    MR. BLATCHLEY: Again, we collected
    21 samples from a number of different facilities and
    22 the idea was to come up with an index test that
    23 would allow us to evaluate how does the microbial
    24 community respond to all of them. So we made no
    0070
    1 attempt to try to characterize or mimic the
    2 differences that exist in the actual receding
    3 waters because I think that the idea there was it
    4 would have complicated the subsequent analysis.
    5 We wanted to set everyone on same playing field so
    6 we could do a direct comparison on how these
    7 things, how the microbial communities responded.
    8
    MR. HARLEY: So, for example, if you

    9 were talking about a discharge which occurred at
    10 the Calumet Waste Water Treatment Plant into the
    11 Calumet River on the southeast side you don't know
    12 six days later where that sample would be in
    13 relationship to where it was discharged, that
    14 would not be a factor in your evaluations, in your
    15 experiment?
    16
    MR. BLATCHLEY: Correct.
    17
    MR. HARLEY: Is it more likely that
    18 the lower numbers achieved in the disinfected
    19 samples on day one would be found closer into the
    20 facility than the samples found on day six?
    21
    MR. BLATCHLEY: That seems
    22 reasonable, yes.
    23
    MR. HARLEY: So if you want to
    24 protect the Chicago area waterways, for example,
    0071
    1 at the point of outfall, then the most relevant
    2 data that we would have from your experiment would
    3 be the data from zero to one as opposed to from
    4 five to six?
    5
    MR. BLATCHLEY: I'm not sure that
    6 I'll comfortable with that suggestion and I'm not
    7 an expert on the Chicago area waterways themselves
    8 in terms of their hydrodynamics, but my
    9 understanding is that the water in the waterways
    10 moves very slowly.
    11
    MR. HARLEY: Throughout the entire
    12 70 plus --
    13
    MR. BLATCHLEY: Again, I'm not an
    14 expert on this, but the little bit of reading I've
    15 done on this does suggests that it does move
    16 pretty slowly.
    17
    MR. HARLEY: Thank you.
    18
    MS. WILLIAMS: Do you know how far
    19 downstream the water travels after six days?
    20
    MR. BLATCHLEY: No, I do not.
    21
    MR. HARLEY: Dr. Blatchley, are
    22 there -- I'm sorry.
    23
    MR. TIPSORD: Go ahead.
    24
    MR. HARLEY: Dr. Blatchley, are
    0072
    1 there other factors in the receding water that may
    2 effect the levels of -- the indicators that you
    3 measured here?
    4
    MR. BLATCHLEY: Yes, I would
    5 imagine.
    6
    MR. HARLEY: And those were not
    7 taken into account, either, in your experiment?
    8
    MR. BLATCHLEY: Again, the idea in
    9 this experiment was to have a consistent index
    10 test that could be used to compare the responses
    11 of the microbial community from many different
    12 waste water treatment facilities. So we wanted to
    13 set that as a standard that all of these tests
    14 were subjected to.
    15
    MR. HARLEY: Thank you.

    16
    MR. ETTINGER: Did you study or
    17 consider what any of the causation elements would
    18 be here that might lead to levels -- Did you look
    19 at the causation that lead you to these numbers?
    20
    MR. BLATCHLEY: No, again, these
    21 were empirical observations.
    22
    MR. ETTINGER: Okay. So sitting
    23 here you have no idea why the numbers went one way
    24 or the another because of the various CAWS?
    0073
    1
    MR. BLATCHLEY: I guess I'm not
    2 quite sure how to answer your question, but I
    3 guess the general answer would be no.
    4
    MS. TIPSORD: Ms. Alexander.
    5
    MS. ALEXANDER: One question to
    6 clarify. Did the level in the sample disinfected
    7 with ultraviolet ever regrow to a point that was
    8 higher than the level in either of the
    9 undisinfected samples?
    10
    MR. BLATCHLEY: I guess in the data
    11 that is present in table three, which is what I
    12 guess you're referring to. I guess I don't see
    13 any examples of where that is so, but, again, the
    14 data that is presented in those tables represent
    15 an average of four experiments that were conducted
    16 in each facility. So I don't know, I don't recall
    17 all the details of all the numbers that went into
    18 this table.
    19
    MS. ALEXANDER: Do you have any
    20 reason to believe that there is data that's not
    21 presented in this table that indicates that the
    22 samples at T equals 144 for the effluent
    23 disinfectant with UV were ever higher than the
    24 samples of undisinfected effluent either with or
    0074
    1 without?
    2
    MR. BLATCHLEY: I'm going to give
    3 you kind of a -- how would I characterize this
    4 response? Part of the motivation for doing this
    5 study was that there had been -- there is concern
    6 that exists in the literature as to the potential
    7 for a process called photoreactivation and another
    8 process called dark repair that would follow UV
    9 irradiation.
    10
    It's also clear in the
    11 literature that microorganisms or microbial
    12 communities can repair sub lethal damage to any
    13 form of stress, at least, in theory. So our goal,
    14 one of our goals in these experiments was to
    15 evaluate to what extent was that repair going to
    16 be important with respect to UV and with respect
    17 to chlorine. In the literature, there does seem
    18 to be for whatever reason, let's say, more concern
    19 associated with photoreactivation and dark repair.
    20 In other words, the repair and recovery process is
    21 more associated with UV than it is with the
    22 similar processes that would accompany

    23 chlorination/dechlorination or virtually any other
    24 disinfectant. So we wanted to explore whether
    0075
    1 that was really a valid concern. And my
    2 interpretation of these data is that repair and
    3 regrowth is important with all disinfection
    4 processes.
    5
    MS. ALEXANDER: Okay. But I need to
    6 refer back to my original question. You have
    7 identified some concerns that you believe exists
    8 in the literature, but I'm asking the question
    9 specifically about the results of your study. And
    10 I'd like to know, did you ever find in any
    11 instance, whether it's reflected in this table or
    12 not, that at T equals 144 the levels in the sample
    13 disinfected with UV were higher than the levels in
    14 the sample that was not disinfected?
    15
    MR. BLATCHLEY: I'll give you I
    16 think the same answer I did before. I don't think
    17 there is any data in table two that would satisfy
    18 that condition and I don't recall any data that
    19 went into the table that would satisfy that
    20 condition either. Does that answer your question?
    21
    MS. ALEXANDER: Yes, that does
    22 answer my question. Thank you.
    23
    MR. ETTINGER: What temperature did
    24 you keep the bottles at during the six days?
    0076
    1
    MR. BLATCHLEY: I don't remember.
    2 I'm going to guess it was nominally room
    3 temperature, but I don't know. Hang on.
    4
    MR. ANDES: It should be in the
    5 report somewhere.
    6
    MR. BLATCHLEY: Each sample was --
    7
    MR. HARLEY: Can we please clarify
    8 what he's reading from for the record.
    9
    MR. BLATCHLEY: Sure. The research
    10 that I'm referring to was sponsored by the Water
    11 Environment Research Foundation and what I'm
    12 looking at is the final report for that project
    13 and it defines -- I brought it with me just
    14 because I thought there might be questions that
    15 come about about the details of the experiments.
    16
    MR. HARLEY: Is that an exhibit at
    17 this point?
    18
    MR. ANDES: I don't believe that it
    19 is. I think it was cited in his testimony and we
    20 can certainly provide it, probably on a disc, for
    21 the record. It's not a problem.
    22
    MR. HARLEY: Thank you.
    23
    MR. BLATCHLEY: If you don't mind,
    24 I'll just read the conditions of incubation. Is
    0077
    1 that okay?
    2
    MR. ETTINGER: Yes.
    3
    MR. BLATCHLEY: Each sample was
    4 placed in a water bath incubator at 25 degrees C

    5 under dark conditions with magnetic stirring.
    6 Does that answer your question?
    7
    MR. ETTINGER: Dark conditions, so
    8 there was no light?
    9
    MR. BLATCHLEY: Correct.
    10
    MS. ALEXANDER: One additional
    11 question on table three, is it fair to say that in
    12 every instance at T equal zero immediately post 7
    13 disinfection the levels of bacteria or indicators
    14 were very substantially reduced at the point of
    15 disinfection?
    16
    MR. BLATCHLEY: What do you mean by
    17 very substantially reduced?
    18
    MS. ALEXANDER: Well, I'll use
    19 examples and we can characterize it if you like,
    20 but looking at Facility B pre-disinfection levels
    21 2.81 times 10 to the 5th, 2.16 times 10 to the
    22 5th, disinfection levels with UV and chlorine
    23 respectfully were 495 and 715. So you go from the
    24 tens of thousands to the hundreds.
    0078
    1
    MR. BLATCHLEY: Right. So that's
    2 roughly three log units of inactivation.
    3
    MS. ALEXANDER: Okay. Same thing
    4 for Facility D. Some numbers in the tens of
    5 thousands to a number in the hundreds and a number
    6 in the tens.
    7
    MR. BLATCHLEY: Yes.
    8
    MS. ALEXANDER: Then Facility A you
    9 have numbers near 10,000 to 55 and 9 respectfully
    10 for UV and chlorination/dechlorination and
    11 Facility C, 2400 and 1900 versus .25 and 2, would
    12 you characterize those as pretty substantial
    13 reductions?
    14
    MR. BLATCHLEY: Three or four log
    15 units of inactivation, yes.
    16
    MS. ALEXANDER: Okay.
    17
    MR. ETTINGER: I have to ask one
    18 really silly question. When you say inactivation
    19 for us guys who don't have quite the same level of
    20 education, does that mean kill or does it send
    21 someone to retirement or vacation?
    22
    MR. BLATCHLEY: What we measure in
    23 the essay that we used to quantify, for example,
    24 coliform bacteria is their ability to reproduce.
    0079
    1 If an organism is dead, it can't reproduce, but
    2 the opposite is not necessarily true. In other
    3 words, if an organism does not have the ability to
    4 reproduce, it does not have to be dead. So what
    5 we're measuring is it's ability to reproduce or
    6 infect a host and the term used to describe that
    7 is inactivation.
    8
    MR. ETTINGER: Inactivation means no
    9 longer reproduces?
    10
    MR. BLATCHLEY: No longer capable of
    11 reproducing or in the case of a virus, capable of

    12 infecting a host.
    13
    MR. ETTINGER: Thanks.
    14
    MS. ALEXANDER: All right. I'm
    15 going to move on now to pre-file question nine,
    16 which concerns conclusion number three on page
    17 nine in which you state in many other developed
    18 countries waste water disinfection is not
    19 practiced. It appears the frequency of these
    20 transmissions associated with water contact is not
    21 substantially different from that in the US where
    22 waste water disinfection is common. What's the
    23 basis for that statement?
    24
    MR. BLATCHLEY: Largely personal
    0080
    1 experience. Does that answer your question?
    2
    MS. ALEXANDER: Yes. So do I
    3 understand correctly then that you've conducted no
    4 research to back you up that conclusion?
    5
    MR. BLATCHLEY: I've never done a
    6 survey myself if that's what you mean.
    7
    MS. ALEXANDER: Are you aware of any
    8 surveys that others have done? I don't mean
    9 personal, but published.
    10
    MR. BLATCHLEY: Yes.
    11
    MS. ALEXANDER: In this specific
    12 question to recreation -- Well, hold on one
    13 second. Are these studies concerning the
    14 frequency of disease transmission associated with
    15 recreational use?
    16
    MR. BLATCHLEY: No. They're related
    17 to -- is disinfection practiced and, if so, how?
    18
    MS. ALEXANDER: Okay. So they're
    19 related to the disinfection component of your
    20 statement, but not to the frequency of disease
    21 transmission component of your statement?
    22
    MR. BLATCHLEY: Correct.
    23
    MS. ALEXANDER: Okay. Do you have
    24 any information regarding the population of
    0081
    1 various water recreation activities in these
    2 countries you referred to relative to the US?
    3
    MR. BLATCHLEY: Do you mean
    4 popularity?
    5
    MS. ALEXANDER: In other words, jet
    6 skiing. Do you have any information on how many
    7 people in these countries referring to jet ski or
    8 boat or engage in any other types of water
    9 recreation their engaged on the CAWS?
    10
    MR. BLATCHLEY: Empirical
    11 observations, again, based on my own experience.
    12
    MS. ALEXANDER: Okay.
    13
    MR. ANDES: Can you expand on that?
    14
    MR. BLATCHLEY: Sure. I
    15 participated in the sport of rowing for about 25
    16 years and part of that experience involved a club
    17 that I was a member of for a year when I lived in
    18 France on the southwest side of Paris and I would

    19 say that the popularity of or, let's say, fraction
    20 of the population that participates in rowing in
    21 France is similar to the fraction of the
    22 population that participates in rowing in the
    23 United States, perhaps even larger.
    24
    And, again, my own personal
    0082
    1 experience -- I don't recall ever after having
    2 rowed for 25 years I don't recall ever getting
    3 sick as a result of that, nor I do know anybody
    4 who got sick as a result of those 25 years that I
    5 would have rowed with them.
    6
    So my own personal experience
    7 suggests that it's not an activity that leads to
    8 people getting sick and water quality where the
    9 bodies of water that I rowed on were not pristine
    10 mountain lakes. Unfortunately, rowing clubs are
    11 often times positioned in places where water
    12 quality is not consistent with a pristine mountain
    13 lake.
    14
    MS. ALEXANDER: So it would be fair
    15 to say that your experience is essentially
    16 personal of rowing, the personal experience that
    17 you are referring to?
    18
    MR. BLATCHLEY: Largely, yes.
    19
    MS. ALEXANDER: Okay. Did you have
    20 occasion to take my measurements of the bacterial
    21 quality or the bacteria content of the water in
    22 which you were rowing on?
    23
    MR. BLATCHLEY: No.
    24
    MS. ALEXANDER: Okay.
    0083
    1
    MS. WILLIAMS: Did you know if there
    2 were undisinfected effluents being discharged in
    3 the water you were rowing on?
    4
    MS. TIPSORD: Ms. Williams, we can't
    5 hear you.
    6
    MS. WILLIAMS: Were there
    7 undisinfected effluents being discharged directly
    8 into the water where you were rowing in France?
    9
    MR. BLATCHLEY: Yes, and elsewhere.
    10
    MS. WILLIAMS: And can you explain
    11 what treatments, technologies were used.
    12
    MR. BLATCHLEY: I believe the forms
    13 of treatment that they used other than
    14 disinfection are similar to what we would use in
    15 the United States.
    16
    MR. ANDES: Secondary treatment.
    17
    MR. BLATCHLEY: Primary secondary
    18 treatment, yes.
    19
    MS. TIPSORD: Can I ask you,
    20 Dr. Blatchley, where have you rowed in France.
    21
    MR. BLATCHLEY: The club that I
    22 rowed -- that I was a member of was on the
    23 southwest side of Paris downstream of Paris along
    24 the Seine. Do you want to know other than that?
    0084

    1
    MS. TIPSORD: Yes, please.
    2
    MR. BLATCHLEY: We participated in
    3 competition at several places in France on the
    4 Seine and actually one time at Versailles at the
    5 palace. I can assure you that the water quality
    6 at the palace at Versailles is not very good.
    7
    MR. ETTINGER: It wasn't in the
    8 1700's either.
    9
    MR. BLATCHLEY: Correct.
    10
    MR. ETTINGER: May I suggest we hold
    11 our next hearing at that location.
    12
    MR. ANDES: No objection.
    13
    MR. ETTINGER: Let me ask a few
    14 more. Are you familiar with waste water practices
    15 in Germany?
    16
    MR. BLATCHLEY: Not in detail, no.
    17
    MR. ETTINGER: Are you familiar with
    18 the Isar River Restoration Plant?
    19
    MR. BLATCHLEY: I read a little bit
    20 about it after last weeks hearing.
    21
    MR. ETTINGER: Do you know if they
    22 disinfect there?
    23
    MR. BLATCHLEY: I believe I read
    24 about it in response to a question that you raised
    0085
    1 and I believe they do, yes.
    2
    MR. ANDES: If I can follow up on
    3 that, what's your understanding of reasons why
    4 they're doing that?
    5
    MR. BLATCHLEY: The assertion that I
    6 made in the report is that in general western
    7 Europe when disinfection of waste water is
    8 practiced, it's practiced when the waste water is
    9 released to either a beach or a shell fish
    10 breeding ground or some other area where direct
    11 human contact is likely. And, I believe, that's
    12 true at the facility that you're discussing.
    13
    MR. ETTINGER: Do you think the
    14 entire Isar River is a beach?
    15
    MR. ANDES: Am I correct that the
    16 plan is to have swimming areas on the Isar River?
    17
    MR. BLATCHLEY: That's my
    18 understanding, but the sum total of what I know
    19 about that facility is what I read on the web.
    20
    MR. ETTINGER: What about Dublin,
    21 Ireland?
    22
    MR. BLATCHLEY: Same thing. I
    23 believe you raised that same thing about that the
    24 facility. I believe the motivation for the use of
    0086
    1 the UV is the same. You're talking about the
    2 Ringsend facility, I believe, it's called.
    3
    MR. ETTINGER: Have you ever seen
    4 the Liffey?
    5
    MR. ANDES: We actually have
    6 information about the Dublin and Munich situation,
    7 which we can provided for the record.

    8
    MR. ETTINGER: How about Milan,
    9 Italy?
    10
    MR. BLATCHLEY: No, I'm sorry.
    11
    MS. ALEXANDER: And just following
    12 up on your statement --
    13
    MS. TIPSORD: Wait, Ms. Alexander.
    14 Let's mark these exhibits first.
    15
    MS. ALEXANDER: I'm sorry.
    16
    MS. WILLIAMS: Before we mark them,
    17 can we have the witness explain why, you know,
    18 what if he reviews them or what the basis is?
    19 Mr. Andes said we have this information, is it the
    20 same information that you reviewed after the last
    21 hearing?
    22
    MR. BLATCHLEY: Actually, during the
    23 last hearing.
    24
    MR. TIPSORD: And you nodded yes?
    0087
    1
    MR. BLATCHLEY: Yes, I'm sorry.
    2
    MS. TIPSORD: I've been handed
    3 WEDECO once over in Munich, which we will mark as
    4 Exhibit Number 96. If there's no objection,
    5 seeing none, it's Exhibit 96.
    6
    MR. ETTINGER: I'm sorry. Is there
    7 a question on now or are we just passing out
    8 exhibits at this point?
    9
    MR. TIPSORD: I'm marking exhibits
    10 right now. Ringsend (SBR) Waste Water Treatment
    11 Plant Overview. This is for Dublin. I will mark
    12 this as Exhibit 97, if there's no objection,
    13 seeing none, it's Exhibit 97. And Ms. William's
    14 were you satisfied with the answer?
    15
    MS. WILLIAMS: Yes.
    16
    MS. TIPSORD: Then there is no
    17 question pending.
    18
    MR. ETTINGER: Just to complete our
    19 travel around the world, are you familiar with
    20 Madrid, Spain, whether they disinfect there?
    21
    MR. BLATCHLEY: I am not aware.
    22
    MR. ETTINGER: Tokyo, Japan?
    23
    MR. ANDES: Is someone planning to
    24 produce evidence to all of this?
    0088
    1
    MR. TIPSORD: His question is
    2 whether he knows if they do disinfecting, not that
    3 they do disinfect.
    4
    MR. ETTINGER: I have not presented
    5 any information, though.
    6
    MR. ANDES: I'm always glad to do
    7 research for you.
    8
    MR. ETTINGER: Exactly. So to
    9 complete my question on this, Tokyo, Japan, have
    10 you looked at Tokyo, Japan?
    11
    MR. BLATCHLEY: No, I have not.
    12
    MR. ETTINGER: Thank you very much.
    13
    MS. DEXTER: Could I just ask one
    14 question? When did you spend time in France?

    15
    MR. BLATCHLEY: It was '95 and '96.
    16 Just to clarify, that's when I was on sabbatical
    17 there, but I've been back to France a number of
    18 times since then.
    19
    MS. ALEXANDER: And just to follow
    20 up on your statement earlier that you, if I
    21 understood you correctly, that you are not aware
    22 of anyone having gotten sick from that you knew
    23 from the activity of rowing, do you have any
    24 reason to believe one way or the other or to know
    0089
    1 whether your fellow rowers were incumono
    2 (phonetic) compromised or in otherwise part of a
    3 sensitive population?
    4
    MR. BLATCHLEY: I was not aware of
    5 anyone that I rowed with who would fit either one
    6 of those categories, but I didn't ask either.
    7
    MS. ALEXANDER: I didn't expect that
    8 you did either. Turning now to your summary of
    9 conclusions, this is -- I'm sorry. Pre-file
    10 question number 10, conclusion number four on page
    11 nine, you make the statement, you're respective of
    12 any measures that are used to control microbial
    13 inputs to the CAWS for municipal waste water
    14 treatment facilities input from other sources
    15 EGCSO's and non-point sources will remain, would
    16 you say that statement is true with respect to wet
    17 weather condition?
    18
    MR. BLATCHLEY: Yes.
    19
    MS. ALEXANDER: Okay. Do you have
    20 any basis to believe that it is true also with
    21 respect to dry weather conditions?
    22
    MR. BLATCHLEY: Yes. The influence
    23 of wet weather events does not end when the rain
    24 stops. So I would guess that, yes, that is true,
    0090
    1 but you need to define what dry weather conditions
    2 are.
    3
    MS. ALEXANDER: Okay. Dry weather
    4 conditions -- Well, I guess one could use a lot of
    5 definitions. Let me ask you, is there a point at
    6 which you believe the contribution of wet weather
    7 is no longer significant to microbial
    8 contamination?
    9
    MR. BLATCHLEY: I'm not sure.
    10
    MS. ALEXANDER: Would, and we're
    11 just using this for purposes of discussion, you
    12 use a time frame, approximately, you know, two
    13 days would you believe that was -- do you have any
    14 reason to believe that would not be an accurate
    15 measure?
    16
    MR. ANDES: He just said he wasn't
    17 sure.
    18
    MS. ALEXANDER: All right.
    19
    MR. TIPSORD: Excuse me,
    20 Ms. Alexander, if I may. I believe Geosyntec, and
    21 if I'm misstating this I apologize, defines dry

    22 weather was no measurable precipitation two days
    23 before or two days after. In that context, can
    24 you answer the question?
    0091
    1
    MR. BLATCHLEY: I don't expect that
    2 the inputs to the Chicago Area Waterway System
    3 will cut off after a dry weather event completely
    4 and let me just use as an example --
    5
    MR. TIPSORD: Do you mean after a
    6 wet weather event?
    7
    MR. BLATCHLEY: Yes, after a wet
    8 weather event. I'm sorry. For example, the town
    9 that I live in, there is a large river, the Wabash
    10 River, that goes between Lafayette and West
    11 Lafayette, if it hasn't rained for a week, does
    12 the dry up? Of course, not. The flow rate in the
    13 river diminishes, but it does not go away
    14 completely. So clearly there are inputs to the
    15 river that are there continuously.
    16
    MR. ETTINGER: The groundwater.
    17
    MR. BLATCHLEY: That would be one of
    18 them, yes.
    19
    MS. ALEXANDER: Are you aware that
    20 approximately 70 percent of the flow to the CAWS
    21 during dry weather comes through the waste water
    22 treatment plants?
    23
    MR. BLATCHLEY: I've read that, yes.
    24
    MS. ALEXANDER: Do you have any
    0092
    1 reason to believe one way or the other that the
    2 inputs that's you've identified -- I should say
    3 the impacts of the inputs you identified, the
    4 CSO's and non-point sources will be significant
    5 two days or following, you know, after two days
    6 following a rain event?
    7
    MR. BLATCHLEY: I'm not sure I'd be
    8 comfortable characterizing how long it would take.
    9
    MS. ALEXANDER: And I'm asking the
    10 question now whether you have any reason to
    11 believe that the effects of a rainfall event in
    12 terms of CSO's and non-point sources would be
    13 significant two days after that rain fall event in
    14 the CAWS, do you have any reason to believe one
    15 way or the other?
    16
    MR. BLATCHLEY: No, I don't have any
    17 reason to believe one way or the other.
    18
    MS. WILLIAMS: Can I ask a follow up
    19 because I think Ms. Alexander misspoke and you
    20 answered it, but I'd like to ask a clarifying
    21 question. I believe she asked you if you knew if
    22 70 percent is the dry weather input from the
    23 treatment plants in this case. Do you know
    24 whether 70 percent is actually the average input
    0093
    1 from the effluent in this system, isn't the dry
    2 weather closer to 100 percent?
    3
    MR. ANDES: It's been testified to

    4 by other parties. He said he doesn't know one way
    5 or the other.
    6
    MS. WILLIAMS: Can we all stipulate
    7 for the record that Ms. Alexander meant to say --
    8
    MS. TIPSORD: You're lowering your
    9 voice. You have to speak up.
    10
    MS. WILLIAMS: So he doesn't have an
    11 opinion about whether 70 percent or 100 percent --
    12
    MR. ANDES: His opinion doesn't
    13 matter.
    14
    MS. WILLIAMS: But you agree with
    15 that?
    16
    MR. ANDES: No, I'm not going to
    17 agree. I'm not going to recharacterize what was
    18 already testified to. What is in the record is in
    19 the record.
    20
    MS. WILLIAMS: I think he just
    21 testified that he read that 70 percent is a dry
    22 weather flow, is that correct?
    23
    MR. BLATCHLEY: I believe that was
    24 the number that I read, yes.
    0094
    1
    MS. WILLIAMS: Okay. So you believe
    2 70 percent is the dry weather flow for the
    3 treatment. Would you agree with me if I were to
    4 tell you that it was closer to 100 percent in dry
    5 weather, would you believe that was accurate?
    6
    MR. BLATCHLEY: Yes, it's going to
    7 be closer, but I don't know how much closer.
    8
    MS. WILLIAMS: That's fine. Thank
    9 you.
    10
    MR. ETTINGER: Let me clarify. You
    11 have not studied the Chicago Area Waterway System?
    12
    MR. BLATCHLEY: Correct.
    13
    MR. ETTINGER: You're familiar
    14 because of your studies on disinfection and these
    15 bottles in the lab?
    16
    MR. BLATCHLEY: Among other things,
    17 yes.
    18
    MR. ETTINGER: But you're not here
    19 as an expert on the flow or anything else that
    20 specifically has to do with the Chicago Area
    21 Waterway System?
    22
    MR. BLATCHLEY: Correct.
    23
    MR. TIPSORD: Mr. Harley.
    24
    MR. HARLEY: Two, I think very
    0095
    1 simpler questions, I hope.
    2
    MS. TIPSORD: Mr. Harley, you need
    3 to speak up.
    4
    MR. HARLEY: I'm sorry. Two simpler
    5 questions, I hope. In terms of the microbial
    6 inputs that you used in your experiments, are
    7 Chicago area municipal waste water facilities
    8 sources of those microbial inputs into the CAWS
    9 during dry weather conditions?
    10
    MR. BLATCHLEY: Yes.

    11
    MR. HARLEY: Are Chicago area
    12 municipal waste water facilities sources of those
    13 microbial inputs during wet weather conditions?
    14
    MR. BLATCHLEY: Yes.
    15
    MR. HARLEY: Thank you.
    16
    MS. ALEXANDER: I'd like to follow
    17 up now referring to page seven of your pre-file
    18 testimony. This is the second full paragraph that
    19 begins with the words the system. It states the
    20 system is defined by the Tunnel and Reservoir
    21 Plan, TARP, has yielded substantial improvements
    22 in water quality in the CAWS. It is likely that
    23 additional water quality improvements will result
    24 in the completeness of the TARP. However, this
    0096
    1 facility will not accomplish complete capture of
    2 waste water from CSO's, therefore, CSO events will
    3 continue to take place in the greater Chicago
    4 area, moreover, non-point source contributions to
    5 the CAWS will be largely uninfected by TARP?
    6 First question, what is the basis for your
    7 statement that CSO events will continue to take
    8 place in the greater Chicago area post TARP.
    9
    MR. BLATCHLEY: I think you had a
    10 pretty graphic illustration about that a week and
    11 a half ago.
    12
    MS. ALEXANDER: Is TARP completed?
    13
    MR. BLATCHLEY: No. I'm going to
    14 guess that it would not matter what stage of
    15 development TARP was in. The volume of water that
    16 was imposed on Chicago during that storm event
    17 would overwhelm any control system. And the point
    18 that I'm trying to make is that you can't
    19 design -- it's not practical to design any
    20 hydrologic control facility that will deal with
    21 all possible events. There's always a risk that
    22 some event will exceed what you've designed for.
    23 Look at New Orleans.
    24
    MR. ETTINGER: Not a particularly
    0097
    1 good example of a well designed system.
    2
    MR. BLATCHLEY: That's true. But
    3 they were content with it for a long time.
    4
    MS. ALEXANDER: Is it your belief
    5 that when TARP is completed there will be fewer
    6 CSO's than there are currently?
    7
    MR. BLATCHLEY: Yes.
    8
    MS. ALEXANDER: Have you taken any
    9 steps to quantify how much less, how many fewer
    10 CSO events there will be upon completion of TARP?
    11
    MR. BLATCHLEY: No.
    12
    MS. ALEXANDER: Okay. Do you have
    13 any basis other than events in the last couple of
    14 weeks to believe one way or the other or to be
    15 able to quantify one way or the other how many CSO
    16 events there will be post TARP completion?
    17
    MR. BLATCHLEY: No, but, again, the

    18 point that it will never be zero.
    19
    MS. ALEXANDER: Okay. Have you
    20 taken any steps, yourself, to quantify other
    21 non-point contributions to the CAWS?
    22
    MR. BLATCHLEY: No.
    23
    MS. ALEXANDER: Are you aware one
    24 way or the other of any quantification that's been
    0098
    1 done of non-point contributions?
    2
    MR. ANDES: We will have other
    3 witnesses on that.
    4
    MS. ALEXANDER: Okay. And I'm
    5 asking Dr. Blatchley if he's aware of any.
    6
    MR. BLATCHLEY: No.
    7
    MS. TIPSORD: Ms. Alexander, if
    8 you're done with that line of questioning we're
    9 going to take about a ten-minute break.
    10
    MS. ALEXANDER: Okay.
    11
    MR. TIPSORD: Let's take ten
    12 minutes.
    13
    (Whereupon, a break was taken
    14
    after which the following
    15
    proceedings were had.)
    16
    MS. TIPSORD: I think we're ready to
    17 go back on the record. Dr. Blatchley, are you
    18 ready?
    19
    MR. BLATCHLEY: Yes.
    20
    MS. TIPSORD: Ms. Alexander?
    21
    MS. ALEXANDER: Yes.
    22
    MS. TIPSORD: Okay.
    23
    MS. ALEXANDER: I'm sorry. Just
    24 give me one moment. I'll ask the question and we
    0099
    1 can locate the statement if we need to, but this
    2 is pre-file question 11 and the question concerns
    3 the January 2007 article. It's Attachment 3 to
    4 Exhibit 93, the study that you co-authored and
    5 published on that date in which you state at the
    6 end in situations where direct human contact is
    7 likely or suggestive of indigenous or
    8 microorganisms that have near -- outfall area is
    9 likely. It appears that the disinfection of
    10 municipal waste water may yield some direct
    11 benefits. That's the statement I am looking to
    12 mark, but do you recognize that as a statement
    13 that you made in that article?
    14
    MR. BLATCHLEY: Yes.
    15
    MS. ALEXANDER: Is this statement
    16 referring to conventional disinfection as you have
    17 defined it in your testimony?
    18
    MR. BLATCHLEY: No. I'm referring
    19 to disinfection that would be more extensive in
    20 terms of the extent of disinfectant exposure.
    21
    MS. ALEXANDER: Are you referring to
    22 disinfection that would be as extensive as the
    23 standards being applied in California that's
    24 discussed in your testimony?

    0100
    1
    MR. BLATCHLEY: The reuse standard?
    2
    MS. ALEXANDER: Yes.
    3
    MR. BLATCHLEY: Potentially.
    4
    MS. ALEXANDER: Are you referring to
    5 a larger universe, a range of disinfection than
    6 that or are you saying purely that the reuse
    7 standard would be beneficial?
    8
    MR. BLATCHLEY: No, I'm not saying
    9 that the reuse standard would be the standard to
    10 use here. What I'm suggesting is that there is a
    11 range of disinfection applications and I would
    12 expect that a more appropriate standard to apply
    13 here for effluent disinfection would be associated
    14 with more extensive inactivation or more extensive
    15 disinfectant exposure than would be required to
    16 meet the proposed standard.
    17
    MS. ALEXANDER: I'm referring now
    18 specifically here to your statement in the
    19 research where you stated it appears that
    20 disinfection of municipal waste water may yield
    21 some direct benefits. I believe you're testifying
    22 now that as one example of that, the disinfection
    23 to the reuse standard would yield some benefits,
    24 is that correct?
    0101
    1
    MR. BLATCHLEY: Yes.
    2
    MS. ALEXANDER: Would disinfection
    3 to a lesser standard than the reuse standard yield
    4 some benefits?
    5
    MR. BLATCHLEY: In the general
    6 sense, yes, but I think you need to ask what is
    7 the extent of that benefit and what is the cost of
    8 that benefit.
    9
    MS. ALEXANDER: What I'm trying to
    10 do, Dr. Blatchley, is just to make sure we
    11 understand what you meant by that statement that
    12 disinfection may yield some direct benefits. Are
    13 you agreeing that disinfection that is less than
    14 disinfection to the reuse standard is included in
    15 that statement?
    16
    MR. BLATCHLEY: Potentially, yes.
    17
    MS. ALEXANDER: Okay. Would
    18 disinfection to the level proposed by IEPA also be
    19 included in that statement that disinfection of
    20 municipal waste water may also yield some direct
    21 benefits?
    22
    MR. BLATCHLEY: In my view, the
    23 disinfectant exposure that would be required to
    24 satisfy that standard would yield a marginal
    0102
    1 improvement in microbial quality.
    2
    MR. ANDES: If I can follow up on
    3 that? Dr. Blatchley, do you stand by your
    4 statement immediately above that in the paragraph,
    5 the conventional disinfection commonly practiced
    6 in the US is probably not as effective in

    7 preventing communicable disease transmission as is
    8 generally assumed?
    9
    MR. BLATCHLEY: I believe that's
    10 true.
    11
    MR. ANDES: Thank you.
    12
    MS. ALEXANDER: But would you also
    13 agree that disinfection to that level may yield
    14 some direct benefit as opposed to no direct
    15 benefits?
    16
    MR. BLATCHLEY: Ideal and absolute,
    17 that's my nature and I would say the benefit would
    18 be greater than zero, yes.
    19
    MS. ALEXANDER: Okay.
    20
    MR. ANDES: If I can follow up on
    21 that? When you talk about the difference between
    22 the reductions that conventional disinfection may
    23 make with regard to fecal levels versus what it
    24 will do to control other pathogens --
    0103
    1
    MR. BLATCHLEY: The issue is how do
    2 coliform bacteria in general, fecal coliform
    3 bacteria compare to microbial pathogens and the
    4 information that I've provided and that's
    5 available widely in the literature make it very
    6 clear that coliform bacteria is more sensitive to
    7 most disinfectants including chlorine and UV and
    8 ozone than are the vast majority of microbial
    9 pathogens.
    10
    MR. ANDES: So is it fair to say
    11 that treating for 400 using conventional
    12 disinfection may not do much to remove pathogens
    13 in the waterway?
    14
    MR. BLATCHLEY: I believe that's
    15 correct.
    16
    MR. ANDES: Thank you.
    17
    MS. TIPSORD: Mr. Harley, you have a
    18 follow up?
    19
    MR. HARLEY: In what time frame?
    20
    MR. BLATCHLEY: Actually, the dose
    21 response data referred to an immediate response.
    22 In other words, if you were to perform this
    23 experiment at the bench and we do that just
    24 because we have much more controlled conditions
    0104
    1 there, then you would measure the viability or
    2 infectivity immediately after exposure. Now,
    3 that's going to require a day of incubation or
    4 something like that, but the point is that you're
    5 measuring immediately. It's not the same thing as
    6 this incubation test as I referred to before.
    7 Does that answer your question?
    8
    MR. HARLEY: If that's the case, why
    9 six days?
    10
    MR. BLATCHLEY: There were a number
    11 of factors that went into six days. Among them,
    12 how many experiments could we complete with the
    13 financial resources that were made available to

    14 us. We wanted to be able to evaluate several
    15 different facilities that had different forms of
    16 treatment that they were using. We wanted to be
    17 able to replicate those samples and we wanted to
    18 perform a period that we thought was meaningful.
    19
    MR. ANDES: Meaningful in the sense
    20 of that the purpose of the test, if I'm correct,
    21 was not to evaluate the immediate effects of
    22 disinfection, but rather to evaluate repair and
    23 regrowth?
    24
    MR. BLATCHLEY: Right. And as you
    0105
    1 can see here what we observe is that after period
    2 of roughly a week that there is not very much to
    3 differentiate the disinfected and the
    4 undisinfected sample. And in some cases it's less
    5 than a week where we get to that case. So it's a
    6 judgement call on our part that we felt if we
    7 incubated for 144 hours or six days that that
    8 would give us most of the information that we
    9 needed.
    10
    MS. TIPSORD: And for the record,
    11 Dr. Blatchley, when you say what we see here you
    12 were pointing to what is Exhibit 95?
    13
    MR. BLATCHLEY: Correct.
    14
    MR. HARLEY: Just one more follow
    15 up. In terms of Exhibit 95 in the context of the
    16 quote in pre-file question 11 when you're
    17 referring to the near outfall area, is it correct
    18 that the most -- the results which would most
    19 commonly replicate near outfall areas are the
    20 results which are located from zero to one day?
    21
    MR. BLATCHLEY: Those are some vague
    22 terms. Clearly, you are going to be closer to the
    23 outfall as you get closer to T equals zero. And,
    24 you know, how close you are to the outfall depends
    0106
    1 on the average velocity in the stream and how long
    2 you allow it to wait. So I'm not sure that I can
    3 define it any more clearly than that. I'd be
    4 guessing.
    5
    MR. HARLEY: And if you're looking
    6 at that period, the zero to one day period, it
    7 would still be your testimony that the reductions
    8 would be nearly marginal?
    9
    MR. BLATCHLEY: Reductions --
    10
    MR. ANDES: In what?
    11
    MR. HARLEY: Microbial pathogens.
    12
    MR. BLATCHLEY: I belive that's
    13 true, yes.
    14
    MR. TIPSORD: Ms. Alexander.
    15
    MS. ALEXANDER: Yes. And just to
    16 follow up with sub question B from question 11, do
    17 you have any reason to believe one way or the
    18 other that people are not engaging in water
    19 recreation near the outfalls?
    20
    MR. BLATCHLEY: No.

    21
    MR. ANDES: So, in other words, you
    22 have no knowledge one way or the other?
    23
    MR. BLATCHLEY: Correct.
    24
    MS. ALEXANDER: And you also have no
    0107
    1 knowledge one way or the other of whether anybody
    2 who is recreating in those locations might ingest
    3 water in the course of their activities?
    4
    MR. BLATCHLEY: Actually, I would
    5 guess that occasionally they do.
    6
    MS. ALEXANDER: Okay. Now, turning
    7 to pre-file question 12, this concerns a further
    8 statement in your conclusions to the January 2000
    9 study that is Attachment 3 that in applying any
    10 disinfectant it is critical the strike a balance
    11 between minimizing risks associated with microbial
    12 pathogens and then associated with disinfection
    13 bi-products and the latest and tocological issues.
    14 And the question is, does UV disinfection create,
    15 to your knowledge, a significant level of
    16 disinfection bi-products?
    17
    MR. BLATCHLEY: I can provide you a
    18 generalization. UV disinfection generally is
    19 regarded as providing fewer disinfection
    20 bi-products than conventional chemical processes
    21 such as chlorination/dechlorination or
    22 ozonization. However, there are circumstances
    23 where there are disinfection bi-products that are
    24 generated by UV or radiation using germicidal UV
    0108
    1 radiation.
    2
    MS. ALEXANDER: Have you done any
    3 work to quantify those levels?
    4
    MR. BLATCHLEY: Yes.
    5
    MS. ALEXANDER: So I would be
    6 correct in understanding that that work has
    7 indicated that those levels are lower than levels
    8 of disinfection bi-product using chlorination?
    9
    MR. BLATCHLEY: Generally.
    10
    MS. ALEXANDER: Can you identify the
    11 work that you have done, are though published peer
    12 review studies?
    13
    MR. BLATCHLEY: Yes and no. So let
    14 me clarify. Yes, we performed a study that was
    15 published in '97 in the journal called Water
    16 Research and I think we presented it at a
    17 conference where we collected waste water effluent
    18 samples, undisinfected waste water effluent
    19 samples and, again, disinfected them at the bench
    20 so we could control disinfectant exposure and then
    21 we perform toxicity studies using an organism
    22 called sariodapia nubia using a fairly standard
    23 toxicity essay and we observed -- we basically did
    24 empirical observations of how these organisms
    0109
    1 responded to the disinfected effluent samples. Is
    2 that what you're asking about?

    3
    I'll just clarify the general
    4 results. All disinfectants that we evaluate which
    5 included chlorine, bromine, ozone and UV have the
    6 ability to influence the toxicological response as
    7 we measured it with the essay that we just
    8 described. In some cases that toxicity response
    9 goes up, meaning it's more toxic. In some cases,
    10 it's goes down and there tends to be not only a
    11 site specific, but also a time dependant
    12 variability that is associated with that. In
    13 other words, you don't get the same response every
    14 day at a facility and if you compare facilities,
    15 you get different responses there as well. But,
    16 in general, we observed less -- there was less
    17 likelihood that there would be an increase in
    18 toxicity associated with UV than there was
    19 associated with either chlorination/dechlorination
    20 or ozone.
    21
    MS. ALEXANDER: Okay.
    22
    MR. ANDES: And we have copies of
    23 that report.
    24
    MS. ALEXANDER: Do you have it now.
    0110
    1 Can we have that marked?
    2
    MR. ANDES: Surely. We also have
    3 copies of the chart, which I believe is Exhibit
    4 95.
    5
    MS. TIPSORD: Correct. Here's the
    6 report and there's Exhibit 95. I've been handed a
    7 handout dealing with waste water effluent toxicity
    8 by Blatchley, et al. I'm looking for a date.
    9
    MR. BLATCHLEY: The upper right.
    10
    MS. TIPSORD: 1997. And I will mark
    11 this as Exhibit 98 if there's no objection.
    12 Seeing none, it's Exhibit 98. And to be clear for
    13 the record, the chart was admitted as Exhibit 95.
    14
    MS. ALEXANDER: I'm not obviously
    15 going to take the whole time to read the study
    16 while we sit here. If you'll give me a moment to
    17 review the abstract and I will continue with my
    18 questions.
    19
    MR. ANDES: It's pretty exciting.
    20
    MS. ALEXANDER: It is.
    21
    MS. TIPSORD: Ms. Alexander, if
    22 you'd like to finish with your questions and come
    23 back to this after lunch after you've had a chance
    24 to review it --
    0111
    1
    MS. ALEXANDER: Yes, that's what I
    2 would like to do. Moving to pre-file question 13,
    3 how prevalent would you say disinfection is in
    4 waste water treatment, generally?
    5
    MR. BLATCHLEY: In the United
    6 States?
    7
    MS. ALEXANDER: In the United
    8 States.
    9
    MR. BLATCHLEY: I'd say it's fairly

    10 common.
    11
    MS. ALEXANDER: Okay. What, if any,
    12 major municipalities in the nation and we'll put a
    13 number on that, population over about a million,
    14 are you aware of in the nation besides Chicago
    15 that are not currently disinfecting their effluent
    16 or are under orders to begin doing so?
    17
    MR. BLATCHLEY: I believe there are
    18 a number of facilities that practice seasonal
    19 disinfection which means for roughly half the year
    20 they don't disinfect.
    21
    MS. ALEXANDER: That's not my
    22 question, though. I mean what municipalities in
    23 that category are you aware of that do not
    24 practice any disinfection and are not under any
    0112
    1 orders to do so?
    2
    MR. BLATCHLEY: I'm not aware of
    3 them.
    4
    MS. ALEXANDER: Okay. Do you have
    5 any knowledge of how many communities in Illinois
    6 are practicing disinfection?
    7
    MR. BLATCHLEY: I do not.
    8
    MS. ALEXANDER: Okay. What method
    9 of disinfection is currently most common in the
    10 country?
    11
    MR. BLATCHLEY: In the United
    12 States.
    13
    MS. ALEXANDER: In the United
    14 States.
    15
    MR. BLATCHLEY: I believe it's
    16 chlorination/dechlorination.
    17
    MS. ALEXANDER: Are there any
    18 facilities that are using ultraviolet?
    19
    MR. BLATCHLEY: Sure.
    20
    MS. ALEXANDER: Okay. And other
    21 than those you've mentioned,
    22 chlorination/dechlorination and ozonization, are
    23 there any other methods of disinfection currently
    24 in use in the United States that you're aware of?
    0113
    1
    MR. BLATCHLEY: I believe there's a
    2 small number of facilities that use bromine and
    3 there are probably some other methods of
    4 disinfections that are out there, but I think
    5 they're just a small fraction.
    6
    MS. ALEXANDER: Okay. That is going
    7 to conclude my questions for now. I'd like to
    8 review the study over lunch as you've suggested,
    9 but we can move on to the other questioners.
    10
    MS. TIPSORD: Okay. That takes us
    11 to the IEPA.
    12
    MS. DIERS: Stephanie Diers from the
    13 Illinois EPA and I'm going to begin with question
    14 one of our pre-file questions. Why would the
    15 conditions of disinfection that are required to
    16 yield a low concentration of viability coliform

    17 not guarantee a low concentration of microbial
    18 pathogens?
    19
    MR. BLATCHLEY: The reason really is
    20 coliform bacteria are generally more sensitive to
    21 disinfectants, meaning chlorine, ozone and UV are
    22 commonly used disinfectants than are most
    23 microbial pathogens -- so the conditions that are
    24 required to accomplish effected inactivation of
    0114
    1 coliform bacteria do not necessarily guarantee
    2 that the microbial pathogens will have been
    3 inactivated effectively.
    4
    MS. DIERS: And, number two, is it
    5 your testimony even if waters are disinfected,
    6 those who come in contact with the disinfected
    7 water can still get sick?
    8
    MR. BLATCHLEY: Yes.
    9
    MS. DIERS: Can you just further
    10 explain that?
    11
    MR. BLATCHLEY: Sure. There is the
    12 potential for microbial pathogens to exist and
    13 that potential will always be there. So if humans
    14 are exposed to those pathogens, then they run the
    15 risk of becoming ill. My understanding is that
    16 the risk that exists right now is low.
    17
    MR. ANDES: With respect to the
    18 CAWS?
    19
    MR. BLATCHLEY: With respect to the
    20 recreational use of the CAWS, yes, I mean
    21 canoeing, kayaking, that sort of thing.
    22
    MS. DEXTER: What's the basis for
    23 that understanding?
    24
    MR. BLATCHLEY: Geosyntec did a risk
    0115
    1 assessment, my reading of that risk assessment
    2 were that the risks were low.
    3
    MS. DEXTER: And that's the risks we
    4 have before us?
    5
    MR. BLATCHLEY: I believe so, yes.
    6
    MS. DIERS: What would a high risk
    7 be in your opinion?
    8
    MR. BLATCHLEY: I'm sorry. I'm
    9 reluctant to provide you with a number because I
    10 just don't know the numbers well enough to know
    11 what high and low would be.
    12
    MS. DIERS: I'll go to question
    13 three. How might chlorination/dechlorination of
    14 UV irradiation be detrimental to water quality in
    15 terms of bacterial composition?
    16
    MR. BLATCHLEY: Again, that refers
    17 to Exhibit 95 and the studies that would relate to
    18 that where we evaluated the long-term response of
    19 the microbial community post disinfection. And
    20 under some circumstances, we observed that water
    21 quality was actually worse post disinfection than
    22 it was if we had done nothing at all.
    23
    MS. DIERS: And when you say some

    24 circumstances, can you give me an example of those
    0116
    1 circumstances?
    2
    MR. BLATCHLEY: We were not able to
    3 establish a cause and effect relationship, again,
    4 these were empirical observations, but, again,
    5 they were empirical observations that were done
    6 with effluent samples from several different waste
    7 water treatment facilities and we observed that in
    8 some cases, water quality, again, was worse post
    9 disinfection than if we had done nothing at all.
    10
    MR. TIPSORD: Mr. Harley, you have a
    11 follow up?
    12
    MR. HARLEY: To clarify, you're
    13 talking about the water qualities in your one
    14 liter samples in your lab, correct?
    15
    MR. BLATCHLEY: Correct.
    16
    MR. HARLEY: You're not talking
    17 about ambient water quality, correct?
    18
    MR. BLATCHLEY: Correct.
    19
    MR. HARLEY: Thank you.
    20
    MR. TIPSORD: Ms. Diers.
    21
    MS. DIERS: I'll go to question
    22 number four. With respect to the conventional
    23 disinfection, what recent research are you
    24 referring to on page five of your pre-file
    0117
    1 testimony?
    2
    MR. BLATCHLEY: That research,
    3 again, is the work that we did that was sponsored
    4 by the Water Environment Research Foundation.
    5
    MR. ANDES: If I can clarify,
    6 reports based on that research are included. One
    7 was Attachment 3 to your testimony, I believe.
    8 There were several reports that were generated as
    9 a result of that research.
    10
    MR. BLATCHLEY: There was three.
    11 There was a journal article, an article that was
    12 published in the Journal of Water and Environment
    13 Research. There was a proceedings article where
    14 there was a conference that was held in Arizona,
    15 the conference was called Disinfection 2005,
    16 because it was held in 2005, where those results
    17 were presented and then there is the report that I
    18 read from earlier, the full report.
    19
    MS. DIERS: And the report is going
    20 to be provided to the group on CD, is that
    21 correct?
    22
    MR. ANDES: Yes.
    23
    MS. DIERS: And are the other two
    24 that you mentioned, are they already in the
    0118
    1 record?
    2
    MR. BLATCHLEY: I think so.
    3
    MR. ANDES: The Water and
    4 Environment Research article was Attachment 3 to
    5 his testimony.

    6
    MS. DIERS: Right.
    7
    MR. ANDES: The other article I am
    8 not sure whether we've provided yet. I know it
    9 was cited, but I have copies of the other article
    10 if that is -- if the people are interested in
    11 that, too, we have copies of that as well.
    12
    MS. TIPSORD: We're interested in
    13 everything and I want to personally thank you in
    14 getting to 100.
    15
    MR. ANDES: I'd be glad to.
    16
    MR. TIPSORD: I've been handed
    17 Effects of Waste Water Disinfection on Human
    18 Health, again, Blatchley, et al.
    19
    MR. BLATCHLEY: Just as a point of
    20 clarification, you're certainly welcome to read
    21 all three of them, but just so you know what
    22 you're getting into. This report, the full
    23 report, is fairly verbose I have to say because I
    24 wrote it. Maybe that's not a cause and effect
    0119
    1 relationship. But, anyway, generally as you move
    2 towards the proceedings article and the referee
    3 journal article, the nature of those publications
    4 is such that there's less room for verbosity, if
    5 that's a word. There are severe restrictions on
    6 what you can publish as you move up the line so
    7 the referee journal article is an abridged version
    8 of this where a lot of the information that is
    9 presented here is simply omitted. There just
    10 wasn't room for it.
    11
    MR. TIPSORD: We will mark Effects
    12 of Waste Water Disinfection on Human Health as
    13 Exhibit 99, if there is no objection. Seeing
    14 none, it's Exhibit 99.
    15
    MS. DIERS: I'll move on to our
    16 pre-file question number five. On page eight of
    17 your pre-file testimony, you state that it is
    18 unlikely that the disinfection process as applied
    19 to CSO's or non-point sources will yield
    20 substantial reductions in the risk of disease
    21 transmission associated with water bourne
    22 microbial pathogens, why is this unlikely?
    23
    MR. BLATCHLEY: The effectiveness of
    24 a disinfection process is going to depend on a
    0120
    1 number of things, including the quality of the
    2 water that you impose on that disinfection
    3 process. In a general sense, the water that is
    4 going to come from a CSO is likely to have poorer
    5 water quality than the effluent that would go into
    6 a disinfection system at a waste water treatment
    7 facility and it's going to have poorer water
    8 quality in terms of a couple general, let's say,
    9 bulk parameters that we might use to characterize
    10 that water quality. That would include the
    11 concentration of particles that's present in the
    12 water as well as the concentration of dissolved

    13 chemicals that might be present in the water.
    14 Irrespective of the disinfectant that you use,
    15 those two things are both going to diminish the
    16 effectiveness of a disinfection process.
    17
    The dissolved chemicals will
    18 represent a source of demand for the disinfectant,
    19 whether that disinfectant is a chemical or an
    20 agent like UV radiation and the particulate matter
    21 that is present is going to provide shelter for
    22 those microorganisms against the disinfectant.
    23
    MS. DIERS: I'm going to go to
    24 number nine. You state in your pre-file testimony
    0121
    1 that chloroform bacteria are poor indicators of
    2 disinfection ethiticity. Is this because they are
    3 easy to kill (or inactivate with chlorine)?
    4
    MR. BLATCHLEY: Yes.
    5
    MS. DIERS: And what would be a good
    6 indicator of disinfection ethiticity?
    7
    MR. BLATCHLEY: Again, as I stated
    8 before, an alternative approach would be to use
    9 perhaps more than one indicator and to use design
    10 criteria that restricts or stipulates a minimum
    11 standard that the actual disinfection must meet in
    12 terms of it's physical characteristics.
    13
    MS. DIERS: I'm going to move on to
    14 our pre-file question 12. Define minimal
    15 improvements in viral composition in control of
    16 protozoic pathogens may also be quite minimal as
    17 you use these phrases on page five of your
    18 pre-file testimony.
    19
    MR. BLATCHLEY: Okay. Just for
    20 reference, this study, the work study, was -- the
    21 central questions that we addressed in that study
    22 were, number one, should we be disinfecting
    23 municipal waste water effluents and then under the
    24 assumption that the answer to that question is
    0122
    1 going to be at least some times yes, then how?
    2
    Those are really kind of the
    3 focal points and so the specific disinfectant that
    4 we examined in that study were
    5 chlorination/dechlorination and UV irradiation.
    6 Chlorine is really not very effective at all for
    7 controlling protozoan pathogens. It's almost
    8 useless for controlling organisms like
    9 criptosperidum parvan or geordialadia (phonetic).
    10 So the effectiveness of chlorine against those
    11 pathogens is really -- I mean it's an issue
    12 because it's so ineffective. On the other hand,
    13 UV is very effective against those specific
    14 microorganisms and UV is a fairly broad spectrum
    15 antimicrobial agent, but there are some
    16 microorganisms, some microbial pathogens that do
    17 not respond well to UV exposure, meaning that they
    18 are not very sensitive to it. They are able to
    19 withstand relatively large doses and still be

    20 viable. And an example of that is adenovirus. So
    21 there are some microbial pathogens and I think
    22 most of them are viral that seem to be resistance
    23 to UV exposure.
    24
    So in the study that we did,
    0123
    1 rather than evaluate human or viral pathogens,
    2 what we did was evaluate the response of some
    3 coliphage. These are bacterial viruses, meaning
    4 that they're viruses that infect human bacterial
    5 hosts rather than human tissues and what we
    6 observed is that under the conditions of
    7 conventional disinfection that correspond to
    8 either chlorination/dechlorination or UV
    9 irradiation that we really didn't accomplish
    10 effective inactivation of those phage in those
    11 experiments. So UV accomplished something on the
    12 order of two log units or two orders of magnitude
    13 inactivation and chlorine, the conditions of
    14 chlorination/dechlorination accomplished something
    15 like one order of magnitude change and when we
    16 talk about control of microbial pathogens, we're
    17 oftentimes interested in four or five log units of
    18 change in the concentration of those pathogens.
    19
    MS. DIERS: I'll move on to 16. On
    20 page five of your pre-file testimony, you state
    21 the populations of microbes in disinfected water
    22 will change with time. Many microbes have the
    23 ability to repair sublethal damage and therefore
    24 can repair post disinfection. What do you mean by
    0124
    1 populations?
    2
    MR. BLATCHLEY: Okay. Just to
    3 clarify, I think the issue is really the microbial
    4 community and how it responds. Have I answered
    5 your question? I'm not sure.
    6
    MS. ALEXANDER: I was going to ask
    7 if you could further explain about what you just
    8 said with the community?
    9
    MR. BLATCHLEY: Again, referring to
    10 Exhibit 95, what we examined was how the microbial
    11 community responded in general and we observed
    12 that some times the microbial community appeared
    13 to be worse post disinfection than if we had done
    14 nothing at all.
    15
    MS. DIERS: Moving on to pre-file
    16 question 17. Are prepared microbes as infectious
    17 as pre-disinfected microbes?
    18
    MR. BLATCHLEY: When I first read
    19 that question, my first thought was great
    20 question. So the general answer is I don't know,
    21 but let me elaborate a little bit. The essay that
    22 we performed to evaluate, for example, how
    23 coliform bacteria responds is one where we look
    24 for their ability to grow. In other words, to
    0125
    1 multiply, to reproduce. And we make no

    2 distinction as to whether they're wounded and able
    3 to reproduce or whether they're 100 percent
    4 healthy, whatever that means.
    5
    We're simply looking for their
    6 ability to reproduce. If we had done this essay
    7 on bacterial pathogens, you could do the same
    8 study. We chose not to. Largely because I didn't
    9 want to be growing bacterial pathogens in my lab,
    10 but if we had done that, then we would have used
    11 very similar essays that looked only for the
    12 ability to reproduce or not reproduce and so that
    13 essay doesn't really tell you anything about the
    14 ability of those organisms to infect, but I would
    15 assume that if it has the ability to reproduce
    16 under the conditions of this essay, then it does
    17 have the ability to infect, but that's an
    18 assumption on my part.
    19
    MS. DIERS: I'm going to skip down
    20 to number 22 on the pre-file questions.
    21
    MR. HARLEY: I'm sorry. Could I ask
    22 a really quick follow up to that? So in terms of
    23 the disease causing potential post disinfection,
    24 we really don't know the answer to that question?
    0126
    1
    MR. BLATCHLEY: I don't.
    2
    MR. HARLEY: Okay.
    3
    MR. ANDES: Let me follow up on
    4 that. You're using as a surrogate bourne
    5 effectivity the ability to reproduce.
    6
    MR. BLATCHLEY: The ability of fecal
    7 coliforms to reproduce.
    8
    MR. ANDES: Right. So the logic is
    9 if they reproduce, they have the ability to
    10 infect?
    11
    MR. BLATCHLEY: Yes.
    12
    MR. ANDES: And you don't know of
    13 any reason why that would be untrue of repaired
    14 fecal coliform versus unrepaired?
    15
    MR. BLATCHLEY: Correct.
    16
    MR. ANDES: Thank you.
    17
    MR. HARLEY: I think the microbial
    18 pathogens --
    19
    MR. BLATCHLEY: Right. I think the
    20 fecal coliforms are largely non-pathogenic. So I
    21 think the question and maybe I'm reading too much
    22 into this, but I think the question is that your
    23 interest is with microbial pathogens that exist in
    24 the water and how their responses might compare to
    0127
    1 those of fecal coliforms, for example. Is that
    2 where you're going?
    3
    MR. HARLEY: Yes.
    4
    MR. BLATCHLEY: And the answer is, I
    5 don't know. We did not investigate any microbial
    6 pathogens and their ability to either repair
    7 subject to this type of essay or their ability to
    8 cause infection in humans which would obviously be

    9 more complicated to investigate.
    10
    MR. HARLEY: So in this situation,
    11 you use fecal coliform to --
    12
    MR. BLATCHLEY: Yes.
    13
    MR. HARLEY: But in other
    14 situations, you made a clear distinction between
    15 fecal coliform and microbial pathogens?
    16
    MR. BLATCHLEY: Yes.
    17
    MR. ANDES: With respect to the
    18 ability to cause illness.
    19
    MR. BLATCHLEY: Yes.
    20
    MS. DIERS: Going back to our
    21 pre-file question 22. On page three and four of
    22 your pre-file testimony you state, although
    23 coliform bacteria are usually plentiful in
    24 untreated municipal waste water, they are easily
    0128
    1 inactivated by waste water disinfectants such as
    2 chlorine, ozone and ultraviolet UV radiation as
    3 compared with many microbial pathogens. As a
    4 result, the conditions of disinfection that are
    5 required to yield a low concentration of viable
    6 coliform bacteria will not guarantee a low
    7 concentration of microbial pathogens. Is there an
    8 indicator organism that if removed will guarantee
    9 a low concentration of microbial pathogens?
    10
    MR. BLATCHLEY: I'm not aware of
    11 one.
    12
    MS. DIERS: Pre-file question number
    13 23. On page four of your pre-filed testimony, you
    14 state disinfection systems used in municipal waste
    15 water treatment applications range from no
    16 infection at all to conditions that accomplished
    17 inactivation of nearly all microbial pathogens.
    18 For purpose of this testimony, the term
    19 conventional disinfection will be used to describe
    20 municipal disinfection systems that are designed
    21 to limit viable coliform concentrations to several
    22 hundred CFU 100 ML. On the spectrum of
    23 disinfection systems use for treatment of
    24 municipal waste water these systems deliver modest
    0129
    1 disinfection doses and accomplish modest microbial
    2 inactivation. If one wants to reduce microbial
    3 pathogens to make the water safer for recreation,
    4 is conventional disinfection a sufficient way to
    5 do those?
    6
    MR. BLATCHLEY: In my opinion, no.
    7
    MS. DIERS: And can you elaborate on
    8 that?
    9
    MR. BLATCHLEY: Again, the results
    10 of the work that we did as well as the results
    11 that have been reported in the literature by
    12 others indicate that the conditions that are
    13 required to accomplish that microbial standard,
    14 for example, 400 CFU's per 100 ML requires fairly
    15 modest exposure to disinfectants. The one result

    16 of that is a fairly modest control of microbial
    17 pathogens because they are less sensitive to the
    18 disinfectants that we use than are the indicator
    19 organisms that are the basis of the regulation.
    20
    MR. TIPSORD: Mr. Harley.
    21
    MR. HARLEY: So would this subject
    22 in your mind that, in fact, a more stringent
    23 numeric limit may be appropriate to control
    24 microbial pathogens?
    0130
    1
    MR. BLATCHLEY: In general, yes. It
    2 depends where you are, what the water use is going
    3 to be, what the water quality issues are. That
    4 sort of thing. But in a general sense, yes.
    5
    MR. HARLEY: So it's possibly that
    6 Illinois EPA proposal of 400 colony forming units
    7 didn't go far enough?
    8
    MR. ANDES: Answer the question
    9 specifically with respect to recreational use.
    10
    MR. BLATCHLEY: It doesn't go far
    11 enough with recreational use, but it also doesn't
    12 go far enough in the sense that it does nothing to
    13 control other sources of microbial pathogens.
    14
    MR. ANDES: When you say it doesn't
    15 go far enough, you're saying that it doesn't
    16 reduce risk, it doesn't reduce pathogen levels?
    17
    MR. BLATCHLEY: It doesn't reduce
    18 the pathogen concentrations as much as we would
    19 like to for this type of exposure.
    20
    MR. ANDES: Let me clarify. The
    21 disinfection requirements that you've talked about
    22 in terms of, say, California are for other uses
    23 such as irrigation.
    24
    MR. BLATCHLEY: Right.
    0131
    1
    MR. ANDES: And they have extensive
    2 disinfections?
    3
    MR. BLATCHLEY: Yes. Far more
    4 extensive than would be required to meet these
    5 standards.
    6
    MR. ANDES: So these standards, in
    7 essence, will do nothing for pathogen reductions
    8 in the CAWS or very little?
    9
    MR. BLATCHLEY: It's not they will
    10 do nothing. It's that they will do very little.
    11
    MR. ANDES: And if you chose the
    12 other level, like in California, it would cost
    13 five to ten times as much?
    14
    MS. WILLIAMS: Objection.
    15
    MR. ANDES: Am I correct?
    16
    MS. WILLIAMS: I object to what he's
    17 testifying.
    18
    MR. ANDES: I'm just asking if
    19 that's your testimony.
    20
    MR. BLATCHLEY: I believe that's
    21 correct. If you were to apply Title 22 standards
    22 here to this sort of disinfection it would cost

    23 five or ten times more.
    24
    MR. TIPSORD: More than --
    0132
    1
    MR. BLATCHLEY: More than would be
    2 required to meet the proposed standards.
    3
    MS. TIPSORD: Thank you.
    4
    MR. HARLEY: But isn't there
    5 something, a standard between 400 colony forming
    6 units and essentially detection limits that might
    7 be appropriate to safeguard recreational users?
    8
    MR. BLATCHLEY: Can we clarify? The
    9 questions is are you asking whether there is some
    10 kind of treatment requirement in between
    11 conventional and extensive inactivation that can
    12 be applied here? You can always pick a number in
    13 between. The question is there something
    14 associated with it in terms of the treatment.
    15
    MR. HARLEY: In response to your
    16 question for clarification, your witnesses
    17 testimony had suggested 400 colony forming units
    18 may not be appropriate because of the microbial
    19 pathogen component of the effluent, but the only
    20 alternative that he really explores in the
    21 testimony is the California standard, which is not
    22 recreational and which is set in a very, very low
    23 level, which is non-detect. Isn't there any
    24 standard in between that might be set as a numeric
    0133
    1 limit that might be appropriate for recreational
    2 use.
    3
    MR. ANDES: It's a numeric limit
    4 that people would treat to.
    5
    MR. BLATCHLEY: I think the way you
    6 qualified that with the word might is how I would
    7 state it. Yes, that's possible, but I don't know
    8 what the number is.
    9
    MR. HARLEY: But 400 colony forming
    10 units, it's your testimony is not enough and the
    11 standard that would be appropriate would more
    12 likely be lower.
    13
    MR. BLATCHLEY: Yes.
    14
    MR. ANDES: So let me ask you this.
    15 Since you laid out the California process as one
    16 that would effectively treat most pathogens, is
    17 there some technology out there that treats
    18 pathogens some but not all the way or are we
    19 talking about you kill them or you don't kill
    20 them?
    21
    MR. BLATCHLEY: Well, there's never
    22 going to be -- Again, disinfection is not the same
    23 thing as sterilization. You're never going to get
    24 to a situation where the risk is completely
    0134
    1 eliminated and a decision is going to be made at
    2 some point as to what is an acceptable risk. Does
    3 that answer your question?
    4
    MR. ANDES: Is there some technology

    5 off the shelf that you would say "Well, here's
    6 moderate disinfection," we've talked about
    7 disinfection conventional and we've talked about
    8 extreme disinfection in California. I think the
    9 question is is there some moderate, medium
    10 disinfection out there?
    11
    MR. BLATCHLEY: Sure. There is an
    12 entire spectrum. It is a continuum effectively.
    13 You can design anywhere in between what would be
    14 conventional disinfection and Title 22
    15 disinfection. You can do it anywhere in that
    16 spectrum.
    17
    MR. ANDES: And the question is
    18 where is that in terms of what would that do to
    19 reduce your pathogen levels, you're still going to
    20 have pathogen levels?
    21
    MR. BLATCHLEY: Sure. And, in
    22 general, as you move towards Title 22, there would
    23 be less risk associated with microbial pathogens?
    24
    MR. HARLEY: Thank you.
    0135
    1
    MR. TIPSORD: Ms. Diers.
    2
    MS. DIERS: Moving on to pre-file
    3 question 28. On page seven of your pre-file
    4 testimony you state, moreover non-point source
    5 contributions to the CAWS will be largely
    6 uneffected by TARP. Therefore, irrespective of
    7 the effluent disinfection constraints that are
    8 imposed on the District's facilities, the
    9 potential for inputs of microbial pathogens from
    10 other sources will still remain. These inputs to
    11 the system will limit the extent to which risk of
    12 disease transmission for microbial pathogens can
    13 be used in the CAWS. My first question is to what
    14 non-point sources are you referring to?
    15
    MR. BLATCHLEY: Well, CSO's to start
    16 with, but just runoff from, you know, whatever,
    17 parking lots, roofs. I suppose there's some grass
    18 areas around as well.
    19
    MS. DIERS: So you consider a CSO a
    20 non-point source?
    21
    MR. BLATCHLEY: No, I'm sorry. I
    22 would not. I would consider a CSO to be a point
    23 source.
    24
    MR. ANDES: Here, when you're
    0136
    1 talking about other sources, you included CSO's?
    2
    MR. BLATCHLEY: Yes. They are
    3 certainly sources of microbial pathogens.
    4
    MS. DIERS: Do non-point source
    5 contributions have the same risk associated with
    6 bacteria as does non-disinfected effluent?
    7
    MR. BLATCHLEY: I don't know.
    8
    MS. DEXTER: Would you expect that
    9 runoff that comes from a roof or a parking lot
    10 would have bacterial or pathogenic composition of
    11 undisinfected sewage effluent?

    12
    MR. BLATCHLEY: I wouldn't drink
    13 either. That's a really difficult question to
    14 answer. I don't have an answer. I'm sorry. I
    15 would not expect, for example, run off from a roof
    16 to be sterile. That's a great way to get sick.
    17
    MS. DEXTER: Comparatively.
    18
    MR. BLATCHLEY: Right. And I don't
    19 know.
    20
    MS. DIERS: Our question 28 and 19
    21 kind of overlap. So I'm just going to ask the
    22 last part of pre-file question 19. Do you believe
    23 generally the presence of CSO's and non-point
    24 sources is sufficient reason to conclude that
    0137
    1 disinfection of waste water treatment plant
    2 effluent is ineffective or unnecessary?
    3
    MR. BLATCHLEY: That contributes to
    4 it.
    5
    MS. DIERS: Okay. Our pre-file
    6 question 29. On page seven of your pre-file
    7 testimony you state, a related point that the
    8 development of disinfection processes for CSO's
    9 and non-point sources represent a difficult
    10 engineering challenge. In your opinion, does the
    11 Illinois EPA proposal require -- Strike that.
    12 Does the Illinois EPA proposal require
    13 disinfection of CSO's and non-point sources?
    14
    MR. BLATCHLEY: Not that I know of.
    15
    MS. DIERS: Would the effluent
    16 disinfection proposal represent a difficult
    17 engineering challenge?
    18
    MR. BLATCHLEY: Conceptually, I
    19 don't think it's -- the extent of disinfectant
    20 exposure that would be required is not an unusual
    21 one, what is unusual is the scale. And my guess
    22 is -- I mean I haven't done the engineering design
    23 on this, but my guess is the complicating issues
    24 associated with a system that would satisfy the
    0138
    1 proposed standard would be largely associated with
    2 this scale and maybe the location and lack of
    3 space and those sorts of issues, but, again, I
    4 have not looked into the details of how it would
    5 be implemented in Chicago.
    6
    MS. DIERS: Just a moment, please.
    7 I think just one more question. I think it
    8 relates back to when we were talking about Exhibit
    9 95. And I'm not sure I was following what you
    10 were saying about the acidic acid. Can you
    11 explain how you were using that again in your
    12 research?
    13
    MR. BLATCHLEY: Sure. The objective
    14 of these experiments was to mimic what would
    15 happen in a receding stream when the effluent is
    16 discharged in a receding stream. So among the
    17 things that the microorganisms that are discharged
    18 to the receding stream are going to see are some

    19 partially reduced substrates. In other words,
    20 food. So what we wanted to do -- and that food is
    21 going to be different in every receding stream,
    22 but for the same reasons that I talked about
    23 before we wanted to come up with a standard essay,
    24 a standard test that we could with all of these
    0139
    1 things that would allow us to compare the results
    2 directly.
    3
    So based on a review of the
    4 literature, we decided that acidic acid at a
    5 concentration of about 15 milligrams per liter
    6 would be not only chemically representative of the
    7 reduced -- partially reduced substrates that would
    8 exist in a receding stream, but also would be
    9 representative of the concentration that we might
    10 expect to see those substrates in receding
    11 streams.
    12
    MS. DIERS: So did you add the
    13 acidic acid substrates to the disinfected samples?
    14
    MR. BLATCHLEY: Yes.
    15
    MS. DIERS: I have nothing further.
    16
    MS. TIPSORD: Are there any
    17 additional questions for Dr. Blatchley?
    18
    MS. ALEXANDER: Not at this time. I
    19 will have some after the lunch break.
    20
    MS. TIPSORD: I didn't want to
    21 necessarily take lunch this early, but we'll take
    22 an hour for lunch. We'll be back at 1:00 and
    23 finish with Dr. Blatchley so we can move on to
    24 Dr. Dorevitch.
    0140
    1 STATE OF ILLINOIS.)
    2
    ) SS.
    3 COUNTY OF COOK )
    4
    5
    6
    7
    I, STEVEN BRICKEY, being a Certified
    8 Shorthand Reporter doing business in the City of
    9 Chicago, Illinois, County of Cook, certify that I
    10 reported in shorthand the proceedings had at the
    11 foregoing hearing of the above-entitled cause.
    12 And I certify that the foregoing is a true and
    13 correct transcript of all my shorthand notes so
    14 taken as aforesaid and contains all the
    15 proceedings had at the said meeting of the
    16 above-entitled cause.
    17
    18
    19
    20 ___________________________
    21 STEVEN BRICKEY, CSR
    CSR NO. 084-004675
    22
    23
    24

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